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Catalog Number Size Price
LS-C800958-100 100 µl (1 mg/ml) $379 
LS-C800958-200 200 µl (1 mg/ml) $421 
PRKCB / PKC-Beta Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
PRKCB / PKC-Beta Antibody - Western blot analysis of Phospho-PKCB (Ser661) antibody expression in heatshock treated HeLa cells lysates. The lane on the right is treated with the antigen-specific peptide.
PRKCB / PKC-Beta Antibody - Western blot analysis of PKCB phosphorylation expression in heatshock treated HeLa whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
PRKCB / PKC-Beta Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
PRKCB / PKC-Beta Antibody - Western blot analysis of Phospho-PKCB (Ser661) antibody expression in heatshock treated HeLa cells lysates. The lane on the right is treated with the antigen-specific peptide.
PRKCB / PKC-Beta Antibody - Western blot analysis of PKCB phosphorylation expression in heatshock treated HeLa whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
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Polyclonal Rabbit anti‑Human PRKCB / PKC‑Beta Antibody (phospho‑Ser661, IF, WB) LS‑C800958

Polyclonal Rabbit anti‑Human PRKCB / PKC‑Beta Antibody (phospho‑Ser661, IF, WB) LS‑C800958

Antibody:
PRKCB / PKC-Beta Rabbit anti-Human Polyclonal (pSer661) Antibody
Application:
IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C800958-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
PRKCB / PKC-Beta Rabbit anti-Human Polyclonal (pSer661) Antibody
Application:
IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
PKC-Beta antibody LS-C800958 is an unconjugated rabbit polyclonal antibody to PKC-Beta (PRKCB) (pSer661) from human. It is reactive with human, mouse and rat. Validated for IF, Peptide-ELISA and WB.
Target
Human PRKCB / PKC-Beta
Synonyms
PRKCB | PKC beta 2 | Pkc betaII | PRKCB2 | Protein kinase c beta | Protein kinase c beta 2 | Protein kinase c beta i | Protein kinase C beta type | Protein kinase C, beta | Protein kinase C, beta 1 | PRKCB1 | Pkc betaI | PKC-beta | PKCB | Pkcbeta | PkcbetaI | PKC-B | PkcbetaII | Protein kinase c beta 1 | Protein kinase c beta II
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Modifications
Unmodified
Immunogen
A synthesized peptide derived from human PKCB around the phosphorylation site of Serine 661.
Epitope
pSer661
Specificity
Phospho-PKCB (Ser661) Antibody detects endogenous levels of PKCB only when phosphorylated at Serine 661.
Applications
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:2000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PRKCB / PKC-Beta
P05771 NM_002738 NP_002729.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/24/2024