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Catalog Number Size Price
LS-C38534-100 100 µg $755 
Antibody

Polyclonal PRKCB / PKC‑Beta Antibody (aa607‑671, WB) LS‑C38534

Polyclonal PRKCB / PKC‑Beta Antibody (aa607‑671, WB) LS‑C38534

Antibody:
Goat Polyclonal to Human PRKCB / PKC-Beta
Application:
ICC, WB, ELISA
Reactivity:
Human, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$755 
LS-C38534-100
100 µg
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Goat Polyclonal to Human PRKCB / PKC-Beta
Application:
ICC, WB, ELISA
Reactivity:
Human, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
PKC-Beta antibody LS-C38534 is an unconjugated goat polyclonal antibody to PKC-Beta (PRKCB) (aa607-671) from human and rat. Validated for ELISA, ICC and WB.
Target
Human PRKCB / PKC-Beta
Synonyms
PRKCB | PKC beta 2 | Pkc betaII | PRKCB2 | Protein kinase c beta | Protein kinase c beta 2 | Protein kinase c beta i | Protein kinase C beta type | Protein kinase C, beta | Protein kinase C, beta 1 | PRKCB1 | Pkc betaI | PKC-beta | PKCB | Pkcbeta | PkcbetaI | PKC-B | PkcbetaII | Protein kinase c beta 1 | Protein kinase c beta II
Host
Goat
Reactivity
Human, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Applications
  • ICC
  • Western blot
  • ELISA
Immunogen
E. coli-derived rhPKCb1 (aa 607-671).
Epitope
aa607-671
Specificity
Recognizes human and rat PKCb1. Selected for its ability to recognize PKCb1 in the applications listed.
Usage
Western Blot: This antibody can be used at 0.1-0.2 ug/ml with the appropriate secondary reagents to detect PKC 1. The detection limit for rhPKC 1 is approximately 0.5 ng/lane under non-reducing and reducing conditions. Immunocytochemistry: This antibody has been used at a concentration of 10 ug/ml to detect PKC 1 in neural progenitor cells. Cells were fixed with PBS containing 4% paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 45 minutes at room temperature. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by Rhodamine RedTM-coupled anti-goat IgG at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing 0.1% BSA. Direct ELISA: This antibody can be used at 0.5-1.0 ug/ml with the appropriate secondary reagents to detect human PKC 1. The detection limit for rhPKC 1 is approximately 0.3 ng/well. In this format, this antibody shows less than 1% cross-reactivity with rhPKC.
Presentation
Lyophilized
Reconstitution
Reconstitute in deionized water
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PRKCB / PKC-Beta
P05771 NM_002738 NP_002729.2

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Requested From: United States
Date Requested: 4/1/2020