PKC-Alpha antibody LS-C17423 is an unconjugated rabbit polyclonal antibody to PKC-Alpha (PRKCA) from human, mouse, rat and other species. Validated for ELISA, IP and WB.
Human PRKCA / PKC-Alpha
Human, Monkey, Mouse, Rat
(tested or 100% immunogen sequence identity)
Protein A purified
- Western blot (1:1000)
- Immunoprecipitation (1:100)
Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Thr638 of human PKC alpha.
Detects human PKC alpha only when phosphorylated at Thr638 and human PKC beta II only when phosphorylated at Thr641. Reacts weakly with phosphorylated PKC beta I and gamma. Recognizes the active form of PKC. Species cross-reactivity: Monkey and mouse. Predicted to cross-react with rat based on sequence homology.
Suitable for use in ELISA, Western Blot and Immunoprecipitation. Western Blot: 1:1000. Immunoprecipitation: 1:100.
10 mM HEPES, pH 7.5, 150 mM NaCl, 0.1 mg/ml BSA, 50% Glycerol, No preservatives added
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.