PKC-Alpha antibody LS-C17430 is an unconjugated rabbit polyclonal antibody to PKC-Alpha (PRKCA) from human, mouse, rat and other species. Validated for ELISA, ICC, IHC and WB.
Rat PRKCA / PKC-Alpha
Rat, Human, Monkey, Mouse, Bovine, Dog, Hamster
(tested or 100% immunogen sequence identity)
- IHC - Paraffin
- IHC - Frozen
- ICC (1:250)
- Western blot (1:250)
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Synthetic peptide corresponding to a distinct C-terminal region, (SYVNPQFVHPILQSAV resides 657-672) from rat protein kinase C alpha. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Panda, Dog, Bovine (100%); Elephant, Horse, Rabbit, Opossum, Platypus (94%); Bat (88%).
Recognizes rat PKCa. Species cross-reactivity: Expected to cross-react with most mammalian species based on known homologies.
Suitable for use in Western Blot, ELISA, Immunocytochemistry and Immunohistochemistry. Immunohistochemistry: 1:250. Fresh or frozen sections. Paraffin embedded and fixed tissues have not been tested. Immunocytochemistry: 1:250. Western Blot: 1:250 using alkaline phosphatase or HRP. Western Blot: >1:250 using ECL. ELISA: >1:250. Optimum dilutions to be determined by researcher.
0.05% Sodium Azide
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
For research use only.