Synthetic peptide corresponding to a distinct C-terminal region, (SYVNPQFVHPILQSAV resides 657-672) from rat protein kinase C alpha. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Panda, Dog, Bovine (100%); Elephant, Horse, Rabbit, Opossum, Platypus (94%); Bat (88%).
Recognizes rat PKCa. Species cross-reactivity: Expected to cross-react with most mammalian species based on known homologies.
Suitable for use in Western Blot, ELISA, Immunocytochemistry and Immunohistochemistry. Immunohistochemistry: 1:250. Fresh or frozen sections. Paraffin embedded and fixed tissues have not been tested. Immunocytochemistry: 1:250. Western Blot: 1:250 using alkaline phosphatase or HRP. Western Blot: >1:250 using ECL. ELISA: >1:250. Optimum dilutions to be determined by researcher.
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
This antibody carries the LSBio 100% Guarantee.