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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PRKACA + PRKACB Antibody (AP) LS‑C510866
PRKACA + PRKACB antibody LS-C510866 is an AP-conjugated rabbit polyclonal antibody to PRKACA + PRKACB from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C510866-100
100 µl
$785
Description
PRKACA + PRKACB antibody LS-C510866 is an AP-conjugated rabbit polyclonal antibody to PRKACA + PRKACB from human, mouse, rat and other species. Validated for WB.
Target
Human PRKACA + PRKACB
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine, Hamster, Pig (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Alkaline Phosphatase. Also available Unconjugated or conjugated with APC, Biotin, FITC, HRP, PE.
Purification
Protein A purified
Modifications
Unmodified
Applications
  • Western blot
  • (applications tested for the base form of this product only)
Immunogen
PRKACA + PRKACB antibody was raised against 16 residue synthetic peptide (KKGSEQESVKEFLAK-C) based upon amino acids 8-22 of the human 41kD alpha isoform of the catalytic subunit of PKA. The immunizing sequence is identical in mouse, rat, hamster, bovine and porcine.
Specificity
Recognizes PKA purified from bovine heart and recombinant human alpha isoform of PKA expressed in CHO cells. Species cross-reactivity: mouse, rat, hamster, bovine and porcine. Cross-reactivity with the beta-isoform of mammalian PKA is possible since it shares identity in 14 of 15 residues.
Presentation
PBS, pH 7.2, No preservatives added
Storage
Store at 4°C.
Restrictions
For research use only.

Popular PRKACA + PRKACB Products

Formalin-fixed and paraffin-embedded human testis tissue reacted with PRKACA Antibody (N-term K82), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Flow Cytometry
Immunohistochemical analysis of PKA C alpha/beta staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Species: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using PKA CAT (Phospho-Thr197) Antibody. The picture on the right is blocked with the phospho peptide.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Western blot of PKA alpha/beta cat antibody
Species: Human, Mouse, Rat
Applications: IHC, Immunofluorescence, Western blot, ELISA
IHC of PKACa (V191) pAb in paraffin-embedded human colon carcinoma.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot

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Requested From: United States
Date Requested: 1/18/2019
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