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Catalog Number	Size	Price
LS-C756673-10	10 µg	$318
LS-C756673-100	100 µg	$470

PRKAB2 / AMPK Beta 2 Antibody - Western blot analysis of AMPK beta 2 using anti-AMPK beta 2 antibody. Electrophoresis was performed on a 10% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysate, Lane 2: human placenta tissue lysate,Lane 3: human 293T whole cell lysate,Lane 4: human A549 whole cell lysate,Lane 5: human A375 whole cell lysate,Lane 6: human A431 whole cell lysate,Lane 7: human U20S whole cell lysate,Lane 8: human K562 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-AMPK beta 2 antigen affinity purified monoclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system.

PRKAB2 / AMPK Beta 2 Antibody - Flow Cytometry analysis of PC-3 cells using anti-AMPK beta 2 antibody. Overlay histogram showing PC-3 cells stained with anti-AMPK beta 2 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-AMPK beta 2 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

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Monoclonal Mouse anti‑Human PRKAB2 / AMPK Beta 2 Antibody (IHC, WB) LS‑C756673

Name: Anti-PRKAB2 / AMPK Beta 2 Antibody | Mouse anti-Human Monoclonal | LSBio
Brand: Lifespan BioSciences
SKU: LS-C756673-10
Price: 318.0000 USD
Availability: InStock

Submit Review LSBio Guarantee

Antibody:

PRKAB2 / AMPK Beta 2 Mouse anti-Human Monoclonal Antibody

Application:

IHC-Fr, ICC, WB, Flo

Reactivity:

Human

Format:

Unconjugated, Unmodified

$318

LS-C756673-10

Toll Free North America

206-374-1102

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

PRKAB2 / AMPK Beta 2 Mouse anti-Human Monoclonal Antibody

Application:

IHC-Fr, ICC, WB, Flo

Reactivity:

Human

Format:

Unconjugated, Unmodified

Specifications

Description

AMPK Beta 2 antibody LS-C756673 is an unconjugated mouse monoclonal antibody to human AMPK Beta 2 (PRKAB2). Validated for Flow, ICC, IHC and WB.

Target

Human PRKAB2 / AMPK Beta 2

Synonyms

PRKAB2 | AMPK beta-2 chain | AMPK beta 2 | AMPK subunit beta-2

See All PRKAB2 / AMPK Beta 2 Antibodies

Host

Mouse

Reactivity

Human (tested or 100% immunogen sequence identity)

Clonality

IgG2b Monoclonal

Conjugations

Unconjugated

Purification

Immunogen affinity purified

Modifications

Unmodified

Immunogen

A synthetic peptide corresponding to a sequence at the N-Terminus of human AMPK beta 2 (56-89 aa DKEFVSWQQDLEDSVKPTQQARPTVIRWSEGGKE), different from the related mouse sequence by three amino acids, and from the related rat sequence by two amino acids.

Specificity

No cross reactivity with other proteins.

Applications

IHC - Frozen (0.5 - 1 µg/ml)
ICC (0.5 - 1 µg/ml)
Western blot (0.1 - 0.5 µg/ml)
Flow Cytometry (1 - 3 µg/10E6 cells)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Usage

Applications should be user optimized.

Presentation

Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500µg/ml.

Storage

After reconstitution, may be stored at 4°C for 1 month. For long-term storage and to avoid freeze-thaw cycles, aliquot and store at -20°C.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.