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Antibodies > PRKAA1 / AMPK Alpha 1 > LS-C808682
 
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Catalog Number Size Price
LS-C808682-100 100 µl (1 mg/ml) $379 
LS-C808682-200 200 µl (1 mg/ml) $421 
PRKAA1 / AMPK Alpha 1 Antibody - 1:100 staining human breast carcinoma tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PRKAA1 / AMPK Alpha 1 Antibody - Staining 293 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
PRKAA1 / AMPK Alpha 1 Antibody - Western blot analysis of AMPK alpha expression in Adriamycin treated COS7 whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
PRKAA1 / AMPK Alpha 1 Antibody - 1:100 staining human breast carcinoma tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PRKAA1 / AMPK Alpha 1 Antibody - Staining 293 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
PRKAA1 / AMPK Alpha 1 Antibody - Western blot analysis of AMPK alpha expression in Adriamycin treated COS7 whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
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Polyclonal Rabbit anti‑Human PRKAA1 / AMPK Alpha 1 Antibody (IHC, IF, WB) LS‑C808682

Polyclonal Rabbit anti‑Human PRKAA1 / AMPK Alpha 1 Antibody (IHC, IF, WB) LS‑C808682

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Antibody:
PRKAA1 / AMPK Alpha 1 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C808682-100
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
PRKAA1 / AMPK Alpha 1 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
AMPK Alpha 1 antibody LS-C808682 is an unconjugated rabbit polyclonal antibody to AMPK Alpha 1 (PRKAA1) from human. It is reactive with human, mouse and rat. Validated for IF, IHC, Peptide-ELISA and WB.
Target
Human PRKAA1 / AMPK Alpha 1
Synonyms
PRKAA1 | ACACA kinase | AMPK alpha 1 | AMPK alpha-1 chain | Acetyl-CoA carboxylase kinase | AMPKa1 | AMPK subunit alpha-1 | AMPK | AMPK1 | Tau-protein kinase PRKAA1 | HMGCR kinase
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Peptide affinity chromatography.
Modifications
Unmodified
Immunogen
A synthesized peptide derived from human AMPK alpha.
Specificity
AMPK alpha Antibody detects endogenous levels of total AMPK alpha.
Applications
  • IHC (1:50 - 1:200)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:2000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PRKAA1 / AMPK Alpha 1
Q13131 NM_006251 NP_006242.5

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Featured Products

Product
PRKAA1 / AMPK Alpha 1 Antibody (phospho‑Ser485) LS‑C34952
Species: Human, Monkey, Mouse, Rat, Hamster
Applications: Western blot
Product
PRKAA1 / AMPK Alpha 1 Antibody (N‑Terminus) LS‑C122645
Species: Drosophila
Applications: IHC, IHC - Frozen, Immunofluorescence, Western blot, Immunoprecipitation, ELISA
PRKAA1 / AMPK Alpha 1 Antibody - IHC of paraffin-embedded ovarian cancer (left) and brain tissues (right) using PRKAA1 mouse monoclonal antibody with DAB staining.
PRKAA1 / AMPK Alpha 1 Antibody (clone 2B7) LS‑C169322
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry, ELISA
PRKAA1 / AMPK Alpha 1 Antibody - Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
IHC‑plus™ PRKAA1 / AMPK Alpha 1 Antibody (aa350‑400) LS‑B14414
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation
Product
PRKAA1 / AMPK Alpha 1 Antibody (N‑Terminus) LS‑C126727
Species: Human, Monkey, Mouse, Rat
Applications: Western blot, Immunoprecipitation, Flow Cytometry
PRKAA1 / AMPK Alpha 1 Antibody - Immunohistochemical analysis of AMPK alpha 1 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
PRKAA1 / AMPK Alpha 1 Antibody (C‑Terminus) LS‑C413155
Species: Human, Mouse, Rat, Chicken
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
See all PRKAA1 / AMPK Alpha 1 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/25/2024
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