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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PPP3R1 / Calcineurin B Antibody LS‑C667722
Calcineurin B antibody LS-C667722 is an unconjugated rabbit polyclonal antibody to human Calcineurin B (PPP3R1). Validated for IHC, IP and WB.
Catalog
Size
Price
LS-C667722-50
50 µl (1 mg/ml)
$235
LS-C667722-100
100 µl (1 mg/ml)
$275
Description
Calcineurin B antibody LS-C667722 is an unconjugated rabbit polyclonal antibody to human Calcineurin B (PPP3R1). Validated for IHC, IP and WB.
Target
Human PPP3R1 / Calcineurin B
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity chromatography
Modifications
Unmodified
Applications
  • IHC (1:50 - 1:200)
  • IHC - Paraffin
  • Western blot (1:500 - 1:2000)
  • Immunoprecipitation (1:50 - 1:100)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Recombinant full length protein of human PPP3R1
Specificity
Recognizes endogenous levels of PPP3R1 protein.
Usage
Western blot analysis of PPP3R1 expression in SW480 (A), MCF7 (B), mouse brain (C), rat testis (D) whole cell lysates. Immunohistochemical analysis of PPP3R1 staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Presentation
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage
Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only.
About PPP3R1 / Calcineurin B
P63098 NM_000945 NP_000936.1

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Images

Immunohistochemistry - Paraffin

Western blot

Immunohistochemistry - Paraffin

Western blot

Immunohistochemistry - Paraffin

Western blot

Immunohistochemistry - Paraffin

Western blot


Requested From: United States
Date Requested: 11/18/2018
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