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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PPP3R1 / Calcineurin B Antibody (aa7‑37) IHC‑plus™ LS‑B11682
Note: This antibody replaces LS-C162092
Calcineurin B antibody LS-B11682 is an unconjugated rabbit polyclonal antibody to human Calcineurin B (PPP3R1). Validated for Flow, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B11682-50
50 µg
$425
Description
Calcineurin B antibody LS-B11682 is an unconjugated rabbit polyclonal antibody to human Calcineurin B (PPP3R1). Validated for Flow, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human PPP3R1 / Calcineurin B
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
KLH-conjugated synthetic peptide from N-terminal region of human PPP3R1.
Epitope
aa7-37
Specificity
Human PPP3R1 / Calcineurin B
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
About PPP3R1 / Calcineurin B
P63098 NM_000945 NP_000936.1

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Images

Immunohistochemistry

Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE)

Western blot

Western blot analysis of PPP3R1 Antibody (N-term) in 293 cell line lysates (35 ug/lane). PPP3R1 (arrow) was detected using the purified Pab.
Western blot analysis of PPP3R1 Antibody (N-term) in 293 cell line lysates (35 ug/lane). PPP3R1 (arrow) was detected using the purified Pab.

Flow Cytometry

PPP3R1 Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
PPP3R1 Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE)

Western blot

Western blot analysis of PPP3R1 Antibody (N-term) in 293 cell line lysates (35 ug/lane). PPP3R1 (arrow) was detected using the purified Pab.
Western blot analysis of PPP3R1 Antibody (N-term) in 293 cell line lysates (35 ug/lane). PPP3R1 (arrow) was detected using the purified Pab.

Flow Cytometry

PPP3R1 Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
PPP3R1 Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE)

Western blot

Western blot analysis of PPP3R1 Antibody (N-term) in 293 cell line lysates (35 ug/lane). PPP3R1 (arrow) was detected using the purified Pab.
Western blot analysis of PPP3R1 Antibody (N-term) in 293 cell line lysates (35 ug/lane). PPP3R1 (arrow) was detected using the purified Pab.

Flow Cytometry

PPP3R1 Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
PPP3R1 Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE)

Western blot

Western blot analysis of PPP3R1 Antibody (N-term) in 293 cell line lysates (35 ug/lane). PPP3R1 (arrow) was detected using the purified Pab.
Western blot analysis of PPP3R1 Antibody (N-term) in 293 cell line lysates (35 ug/lane). PPP3R1 (arrow) was detected using the purified Pab.

Flow Cytometry

PPP3R1 Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
PPP3R1 Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Requested From: United States
Date Requested: 11/16/2018
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