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Catalog Number Size Price
LS-C377488-50 50 µg $294 
LS-C377488-100 100 µg $360 
PPP2R4 Antibody - Immunohistochemistry of paraffin-embedded human testis tissue using antibody at dilution of 1:100.
PPP2R4 Antibody - IHC image of PPP2R4 Antibody diluted at 1:1000 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
PPP2R4 Antibody - IHC image of PPP2R4 Antibody diluted at 1:1000 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
PPP2R4 Antibody - Immunofluorescence staining of Hela cells with PPP2R4 Antibody at 1:333, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
PPP2R4 Antibody - Western Blot Positive WB detected in: SH-SY5Y whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate, K562 whole cell lysate All lanes: PPP2R4 antibody at 3.3µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 41, 37, 34, 32 kDa Observed band size: 41 kDa
PPP2R4 Antibody - Western blot. All lanes: PPP2R4 antibody at 10 ug/ml. Lane 1: K562 whole cell lysate. Lane 2: MCF7 whole cell lysate. Lane 3: A431 whole cell lysate. Lane 4: Raji whole cell lysate. Secondary Goat polyclonal to Rabbit IgG at 1:10000 dilution. Predicted band size: 41 kDa. Observed band size: 41 kDa.
PPP2R4 Antibody - Immunohistochemistry of paraffin-embedded human testis tissue using antibody at dilution of 1:100.
PPP2R4 Antibody - IHC image of PPP2R4 Antibody diluted at 1:1000 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
PPP2R4 Antibody - IHC image of PPP2R4 Antibody diluted at 1:1000 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
PPP2R4 Antibody - Immunofluorescence staining of Hela cells with PPP2R4 Antibody at 1:333, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
PPP2R4 Antibody - Western Blot Positive WB detected in: SH-SY5Y whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate, K562 whole cell lysate All lanes: PPP2R4 antibody at 3.3µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 41, 37, 34, 32 kDa Observed band size: 41 kDa
PPP2R4 Antibody - Western blot. All lanes: PPP2R4 antibody at 10 ug/ml. Lane 1: K562 whole cell lysate. Lane 2: MCF7 whole cell lysate. Lane 3: A431 whole cell lysate. Lane 4: Raji whole cell lysate. Secondary Goat polyclonal to Rabbit IgG at 1:10000 dilution. Predicted band size: 41 kDa. Observed band size: 41 kDa.
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Polyclonal Rabbit anti‑Human PPP2R4 Antibody (IHC, WB) LS‑C377488

Polyclonal Rabbit anti‑Human PPP2R4 Antibody (IHC, WB) LS‑C377488

Antibody:
PPP2R4 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$294
LS-C377488-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
PPP2R4 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
PPP2R4 antibody LS-C377488 is an unconjugated rabbit polyclonal antibody to human PPP2R4. Validated for ELISA, IHC and WB.
Target
Human PPP2R4
Synonyms
PPP2R4 | PP2A phosphatase activator | PP2A subunit B isoform PR53 | PTPA | PP2A | PP2A, subunit B, PR53 isoform | PR53
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with HRP, FITC, Biotin.
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Recombinant human Serine/threonine-protein phosphatase 2A activator protein.
Specificity
Human PPP2R4
Applications
  • IHC
  • IHC - Paraffin (1:20 - 1:200)
  • Western blot (1:500 - 1:2000)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
Storage
Short term: -20°C; Long term: -80°C; Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PPP2R4
Q15257 NM_021131 NP_066954.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/25/2024