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Catalog Number Size Price
LS-C745300-25 25 µl (1 mg/ml) $275 
PPP1R13L / iASPP Antibody - Affinity Purified anti-iASPP antibody shows strong cytoplasmic and membranous staining of myocytes in human heart tissue. Tissue was formalin-fixed and paraffin embedded. Brown color indicates presence of protein, blue color shows cell nuclei.
PPP1R13L / iASPP Antibody - Western blot using the affinity purified anti-iASPP antibody shows detection of a band at ~100 kDa (arrowhead) corresponding to isoform 1 of iASPP in MCF7 whole cell lysates. Preincubation with immunizing peptide blocks specific band staining (data not shown). Approximately 35 ug of lysate was separated by 4-20% Tris Glycine SDS-PAGE. After blocking, the membrane was probed with the primary antibody diluted to 1:1,500 in 5% BLOTTO/PBS overnight at 4°C. The membrane was washed and reacted with a 1:10,000 dilution of conjugated Gt-a-Rabbit IgG [H&L] for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers. Fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR.  Other detection systems will yield similar results.
PPP1R13L / iASPP Antibody - Affinity Purified anti-iASPP antibody shows strong cytoplasmic and membranous staining of myocytes in human heart tissue. Tissue was formalin-fixed and paraffin embedded. Brown color indicates presence of protein, blue color shows cell nuclei.
PPP1R13L / iASPP Antibody - Western blot using the affinity purified anti-iASPP antibody shows detection of a band at ~100 kDa (arrowhead) corresponding to isoform 1 of iASPP in MCF7 whole cell lysates. Preincubation with immunizing peptide blocks specific band staining (data not shown). Approximately 35 ug of lysate was separated by 4-20% Tris Glycine SDS-PAGE. After blocking, the membrane was probed with the primary antibody diluted to 1:1,500 in 5% BLOTTO/PBS overnight at 4°C. The membrane was washed and reacted with a 1:10,000 dilution of conjugated Gt-a-Rabbit IgG [H&L] for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers. Fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR.  Other detection systems will yield similar results.
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Polyclonal Rabbit anti‑Human PPP1R13L / iASPP Antibody (IHC, WB) LS‑C745300

Polyclonal Rabbit anti‑Human PPP1R13L / iASPP Antibody (IHC, WB) LS‑C745300

Antibody:
PPP1R13L / iASPP Rabbit anti-Human Polyclonal Antibody
Application:
IHC, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$275
LS-C745300-25
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
PPP1R13L / iASPP Rabbit anti-Human Polyclonal Antibody
Application:
IHC, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
IASPP antibody LS-C745300 is an unconjugated rabbit polyclonal antibody to human iASPP (PPP1R13L). Validated for ELISA, IHC and WB.
Target
Human PPP1R13L / iASPP
Synonyms
PPP1R13L | IASPP | Inhibitor of ASPP protein | NFkB-interacting protein 1 | NKIP1 | RAI4 | RAI | Retinoic acid induced 4 | NFkB interacting protein 1 | PPP1R13BL | Protein iASPP | RelA-associated inhibitor | PPP1R13B-like protein
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 775-800 of human iASPP (isoform 1) protein.
Specificity
A BLAST analysis was used to suggest cross reactivity with iASPP proteins from human, dog and mouse sources. Expect reactivity against both isoform 1 and isoform 2. Partial reactivity may occur against iASPP from bovine. Reactivity against homologues from other sources is not known.
Applications
  • IHC (1:1000 - 1:5000)
  • Western blot (1:1000 - 1:5000)
  • ELISA (1:40000 - 1:160000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PPP1R13L / iASPP
Q8WUF5 NM_006663 NP_006654.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/17/2021