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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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POU4F3 / BRN3C Antibody (clone 5B8) LS‑C197954
BRN3C antibody LS-C197954 is an unconjugated mouse monoclonal antibody to human BRN3C (POU4F3). Validated for ELISA, IF, RNAi and WB.
Catalog
Size
Price
LS-C197954-100
100 µg
$355

Popular POU4F3 / BRN3C Products

POU4F3 antibody ARP33065_T100-NP_002691-POU4F3 (POU domain, class 4, transcription factor 3) Antibody was used in IHC to stain formalin-fixed, paraffin-embedded human kidney.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Chicken, Xenopus, Zebrafish
Applications: IHC, IHC - Paraffin, Western blot
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
Immunohistochemistry of paraffin-embedded human placenta tissue at dilution 1:100
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
POU4F3 antibody ARP33065_T100-NP_002691-POU4F3 (POU domain, class 4, transcription factor 3) Antibody was used in IHC to stain formalin-fixed, paraffin-embedded human kidney.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Chimpanzee, Gibbon, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Chicken, Xenopus, Zebrafish
Applications: IHC, IHC - Paraffin, Western blot

Product Description

BRN3C antibody LS-C197954 is an unconjugated mouse monoclonal antibody to human BRN3C (POU4F3). Validated for ELISA, IF, RNAi and WB.

Specifications

Target
Human POU4F3 / BRN3C
Synonyms
POU4F3, Brain-3C, BRN3C, POU class 4 homeobox 3, Brn-3C, DFNA15
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal [5B8]
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Applications
  • Immunofluorescence (10 µg/ml)
  • Western blot
  • ELISA
  • RNA interference
Immunogen
POU4F3 / BRN3C antibody was raised against pOU4F3 (NP_002691, aa100-190 partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human POU4F3 / BRN3C
Usage
RNAi Knockdown: Antibody validated. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Transfected lysate, Recombinant protein.
Presentation
PBS, pH 7.4
Storage
Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
Restrictions
For research use only.
About POU4F3 / BRN3C
POU4F3 / BRN3C is a member of the POU-domain family of transcription factors. POU-domain proteins have been observed to play important roles in control of cell identity in several systems. This protein is found in the retina and may play a role in determining or maintaining the identities of a small subset of visual system neurons. Defects in this gene are the cause of non-syndromic sensorineural deafness autosomal dominant type 15. Q15319 NM_002700 NP_002691.1


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Images

Immunofluorescence

Immunofluorescence of monoclonal antibody to POU4F3 on HeLa cell. [antibody concentration 10 ug/ml]
Immunofluorescence of monoclonal antibody to POU4F3 on HeLa cell. [antibody concentration 10 ug/ml]

Western blot

POU4F3 monoclonal antibody (M01), clone 5B8 Western Blot analysis of POU4F3 expression in HeLa NE.
POU4F3 monoclonal antibody (M01), clone 5B8 Western Blot analysis of POU4F3 expression in HeLa NE.

Western blot

Western Blot analysis of POU4F3 expression in transfected 293T cell line by POU4F3 monoclonal antibody (M01), clone 5B8.Lane 1: POU4F3 transfected lysate(37.1 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of POU4F3 expression in transfected 293T cell line by POU4F3 monoclonal antibody (M01), clone 5B8.Lane 1: POU4F3 transfected lysate(37.1 KDa).Lane 2: Non-transfected lysate.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged POU4F3 is approximately 0.1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged POU4F3 is approximately 0.1 ng/ml as a capture antibody.

RNA interference

Western blot analysis of POU4F3 over-expressed 293 cell line, cotransfected with POU4F3 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with POU4F3 monoclonal antibody (M01), clone 5B8 . GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western blot analysis of POU4F3 over-expressed 293 cell line, cotransfected with POU4F3 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with POU4F3 monoclonal antibody (M01), clone 5B8 . GAPDH ( 36.1 kDa ) used as specificity and loading control.

Immunofluorescence

Immunofluorescence of monoclonal antibody to POU4F3 on HeLa cell. [antibody concentration 10 ug/ml]
Immunofluorescence of monoclonal antibody to POU4F3 on HeLa cell. [antibody concentration 10 ug/ml]

Western blot

POU4F3 monoclonal antibody (M01), clone 5B8 Western Blot analysis of POU4F3 expression in HeLa NE.
POU4F3 monoclonal antibody (M01), clone 5B8 Western Blot analysis of POU4F3 expression in HeLa NE.

Western blot

Western Blot analysis of POU4F3 expression in transfected 293T cell line by POU4F3 monoclonal antibody (M01), clone 5B8.Lane 1: POU4F3 transfected lysate(37.1 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of POU4F3 expression in transfected 293T cell line by POU4F3 monoclonal antibody (M01), clone 5B8.Lane 1: POU4F3 transfected lysate(37.1 KDa).Lane 2: Non-transfected lysate.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged POU4F3 is approximately 0.1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged POU4F3 is approximately 0.1 ng/ml as a capture antibody.

RNA interference

Western blot analysis of POU4F3 over-expressed 293 cell line, cotransfected with POU4F3 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with POU4F3 monoclonal antibody (M01), clone 5B8 . GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western blot analysis of POU4F3 over-expressed 293 cell line, cotransfected with POU4F3 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with POU4F3 monoclonal antibody (M01), clone 5B8 . GAPDH ( 36.1 kDa ) used as specificity and loading control.

Immunofluorescence

Immunofluorescence of monoclonal antibody to POU4F3 on HeLa cell. [antibody concentration 10 ug/ml]
Immunofluorescence of monoclonal antibody to POU4F3 on HeLa cell. [antibody concentration 10 ug/ml]

Western blot

POU4F3 monoclonal antibody (M01), clone 5B8 Western Blot analysis of POU4F3 expression in HeLa NE.
POU4F3 monoclonal antibody (M01), clone 5B8 Western Blot analysis of POU4F3 expression in HeLa NE.

Western blot

Western Blot analysis of POU4F3 expression in transfected 293T cell line by POU4F3 monoclonal antibody (M01), clone 5B8.Lane 1: POU4F3 transfected lysate(37.1 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of POU4F3 expression in transfected 293T cell line by POU4F3 monoclonal antibody (M01), clone 5B8.Lane 1: POU4F3 transfected lysate(37.1 KDa).Lane 2: Non-transfected lysate.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged POU4F3 is approximately 0.1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged POU4F3 is approximately 0.1 ng/ml as a capture antibody.

RNA interference

Western blot analysis of POU4F3 over-expressed 293 cell line, cotransfected with POU4F3 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with POU4F3 monoclonal antibody (M01), clone 5B8 . GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western blot analysis of POU4F3 over-expressed 293 cell line, cotransfected with POU4F3 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with POU4F3 monoclonal antibody (M01), clone 5B8 . GAPDH ( 36.1 kDa ) used as specificity and loading control.

Immunofluorescence

Immunofluorescence of monoclonal antibody to POU4F3 on HeLa cell. [antibody concentration 10 ug/ml]
Immunofluorescence of monoclonal antibody to POU4F3 on HeLa cell. [antibody concentration 10 ug/ml]

Western blot

POU4F3 monoclonal antibody (M01), clone 5B8 Western Blot analysis of POU4F3 expression in HeLa NE.
POU4F3 monoclonal antibody (M01), clone 5B8 Western Blot analysis of POU4F3 expression in HeLa NE.

Western blot

Western Blot analysis of POU4F3 expression in transfected 293T cell line by POU4F3 monoclonal antibody (M01), clone 5B8.Lane 1: POU4F3 transfected lysate(37.1 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of POU4F3 expression in transfected 293T cell line by POU4F3 monoclonal antibody (M01), clone 5B8.Lane 1: POU4F3 transfected lysate(37.1 KDa).Lane 2: Non-transfected lysate.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged POU4F3 is approximately 0.1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged POU4F3 is approximately 0.1 ng/ml as a capture antibody.

RNA interference

Western blot analysis of POU4F3 over-expressed 293 cell line, cotransfected with POU4F3 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with POU4F3 monoclonal antibody (M01), clone 5B8 . GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western blot analysis of POU4F3 over-expressed 293 cell line, cotransfected with POU4F3 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with POU4F3 monoclonal antibody (M01), clone 5B8 . GAPDH ( 36.1 kDa ) used as specificity and loading control.

Requested From: United States
Date Requested: 11/13/2018

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