BRN1 antibody LS-C109736 is an unconjugated rabbit polyclonal antibody to BRN1 (POU3F3) from human, mouse and rat. Validated for WB.
Human POU3F3 / BRN1
Human, Mouse, Rat
(tested or 100% immunogen sequence identity)
Also available conjugated with Biotin
- Western blot (0.2 - 1 µg/ml)
- (applications tested for the base form of this product only)
POU3F3 / BRN1 antibody was raised against synthetic peptide located between aa35-84 of human POU3F3 (P20264, NP_006227). Percent identity by BLAST analysis: Human, Mouse, Rat, Opossum (100%).
- Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C109736. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Lyophilized from PBS, 0.09% sodium azide, 2% sucrose
Centrifuge the vial prior to opening. Reconstitute with sterile distilled water to a concentration of 1 mg/ml. Vortex and centrifuge again.
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
For research use only.