SGK196 antibody LS-C81507 is an unconjugated rabbit polyclonal antibody to SGK196 (POMK) from human, mouse, monkey and other species. Validated for WB.
Human, Monkey, Mouse, Rabbit
(tested or 100% immunogen sequence identity)
Also available conjugated with Biotin
POMK / SGK196 antibody was raised against synthetic peptide located between aa74-123 of human SGK196 (Q9H5K3, NP_115613). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Mouse, Panda, Rabbit (100%); Gibbon, Rat, Bovine, Opossum, Guinea pig, Lizard (92%); Hamster, Chicken, Platypus, Xenopus (85%).
ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
PBS, 0.09% sodium azide, 2% sucrose
Short term: Store at 2-8°C. Long term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.