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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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POMC / Proopiomelanocortin Antibody (aa256‑267) LS‑C55321
Proopiomelanocortin antibody LS-C55321 is an unconjugated goat polyclonal antibody to human Proopiomelanocortin (POMC). Validated for IHC, Peptide-ELISA and WB.
Catalog
Size
Price
LS-C55321-100
100 µg (0.5 mg/ml)
$405

Popular POMC / Proopiomelanocortin Products

Western blot analysis of extracts of various cells.
Species: Human, Mouse, Rat
Applications: Western blot
IHC of paraffin-embedded Human lymph node tissue using anti-POMC mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min).
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
Immunohistochemistry of Human breast cancer using POMC Polyclonal Antibody at dilution of 1:30.
Species: Human, Mouse
Applications: IHC, Western blot, ELISA

Product Description

Proopiomelanocortin antibody LS-C55321 is an unconjugated goat polyclonal antibody to human Proopiomelanocortin (POMC). Validated for IHC, Peptide-ELISA and WB.

Specifications

Target
Human POMC / Proopiomelanocortin
Synonyms
POMC, Alpha-MSH, ACTH, Adrenocorticotropin, Adrenocorticotropic hormone, Beta-LPH, CLIP, Gamma-MSH, Lipotropin gamma, LPH, Melanotropin alpha, Met-enkephalin, Melanotropin beta, NPP, Pro-ACTH-endorphin, Lipotropin beta, Melanotropin gamma, MSH, Proopiomelanocortin, Beta-endorphin, Beta-MSH, Corticotropin-lipotropin, Gamma-LPH, POC, Pro-opiomelanocortin
Host
Goat
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Monkey (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (2.5 µg/ml)
  • Western blot (1:32000) & (0.01 - 0.03 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:32000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic peptide C-NAIIKNAYKKGE from the C-terminus of human POMC (NP_000930.1; NP_001030333.1). Percent identity with other species by BLAST analysis: Human (100%), Gorilla (100%), Gibbon (100%), Baboon (92%), Monkey (92%), Marmoset (92%), Elephant (92%), Frog (92%), Mouse (83%), Rat (83%), Sheep (83%), Panda (83%), Dog (83%), Bovine (83%).
Blocking Peptide
LS-E25863 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C55321. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa256-267
Specificity
Human POMC. Both transcript variants encode the same protein. In Western blot, this product appears to react with the full length precursor protein only.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About POMC / Proopiomelanocortin
POMC / Proopiomelanocortin is a polypeptide hormone precursor that undergoes extensive, tissue-specific, post-translational processing via cleavage by subtilisin-like enzymes known as prohormone convertases. There are eight potential cleavage sites within the polypeptide precursor and, depending on tissue type and the available convertases, processing may yield as many as ten biologically active peptides involved in diverse cellular functions. P01189 NM_000939 NP_000930.1


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Images

Western blot

Antibody (0.01 ug/ml) staining of NIH 3T3 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Antibody (0.01 ug/ml) staining of NIH 3T3 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

Antibody (0.01 ug/ml) staining of NIH 3T3 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Antibody (0.01 ug/ml) staining of NIH 3T3 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

Antibody (0.01 ug/ml) staining of NIH 3T3 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Antibody (0.01 ug/ml) staining of NIH 3T3 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

Antibody (0.01 ug/ml) staining of NIH 3T3 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Antibody (0.01 ug/ml) staining of NIH 3T3 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Requested From: United States
Date Requested: 11/14/2018

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