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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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POLR3D Antibody (aa175‑225) LS‑C289046 Best Seller
POLR3D antibody LS-C289046 is an unconjugated rabbit polyclonal antibody to human POLR3D. Validated for IF, IHC, IP and WB.
Catalog
Size
Price
LS-C289046-100
100 µl (0.2 mg/ml)
$395

Popular POLR3D Products

Detection of Human POLR3D by Immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-POLR3D used at a dilution of 1:100.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence
Antibody
Species: Human
Applications: Western blot, ELISA
Antibody
Species: Human
Applications: Western blot, ELISA
Antibody
Species: Human, Mouse
Applications: Western blot, ELISA

Product Description

POLR3D antibody LS-C289046 is an unconjugated rabbit polyclonal antibody to human POLR3D. Validated for IF, IHC, IP and WB.

Specifications

Target
Human POLR3D
Synonyms
POLR3D, Protein BN51, RNA polymerase III subunit C4, RPC4, RPC53 homolog, TSBN51, BN51, BN51T, RPC53
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:200 - 1:1000)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:2000 - 1:10000)
  • Immunoprecipitation (10 µg/mg lysate)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Epitope
aa175-225
Specificity
Region between residue 175 and 225 of human DNA-directed RNA polymerase III subunit D using the numbering given in entry NP_001713.2 (GeneID 661).
Usage
Immunohistochemistry: Antigen retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. Human controls: Breast Carcinoma, Prostate Carcinoma.
Manufacturer
Bethyl Laboratories, Inc.
Presentation
TBS, 0.09% Sodium Azide, 0.1% BSA
Storage
Store at 2°C to 8°C for up to 1 year.
Restrictions
For research use only.
About POLR3D
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. P05423 NM_001722 NP_001713.2


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Immunohistochemistry

Detection of Human POLR3D by Immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-POLR3D used at a dilution of 1:1000 (0.2 ug/ml). Detection: DAB.
Detection of Human POLR3D by Immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-POLR3D used at a dilution of 1:1000 (0.2 ug/ml). Detection: DAB.

Immunohistochemistry

Detection of Human POLR3D by Immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-POLR3D used at a dilution of 1:1000 (0.2 ug/ml). Detection: DAB.
Detection of Human POLR3D by Immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-POLR3D used at a dilution of 1:1000 (0.2 ug/ml). Detection: DAB.

Immunohistochemistry

Detection of Human POLR3D by Immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-POLR3D used at a dilution of 1:1000 (0.2 ug/ml). Detection: DAB.
Detection of Human POLR3D by Immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-POLR3D used at a dilution of 1:1000 (0.2 ug/ml). Detection: DAB.

Immunohistochemistry

Detection of Human POLR3D by Immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-POLR3D used at a dilution of 1:1000 (0.2 ug/ml). Detection: DAB.
Detection of Human POLR3D by Immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-POLR3D used at a dilution of 1:1000 (0.2 ug/ml). Detection: DAB.

Requested From: United States
Date Requested: 11/12/2018

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