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Catalog Number Size Price
LS-C750634-20 20 µl $287 
LS-C750634-50 50 µl $341 
LS-C750634-100 100 µl $431 
LS-C750634-200 200 µl $539 
POLR2A / RNA polymerase II Antibody - Western blot analysis of extracts of MCF7 cells, using Phospho-POLR2A-S5 antibody at 1:1000 dilution. MCF7 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37℃ for 1 hour. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. Blocking buffer: 3% BSA.An ECL Kit was used for detection and the exposure time was 1s.

Polyclonal Rabbit anti‑Human POLR2A / RNA polymerase II Antibody (phospho‑Ser5, WB) LS‑C750634

Polyclonal Rabbit anti‑Human POLR2A / RNA polymerase II Antibody (phospho‑Ser5, WB) LS‑C750634

Antibody:
POLR2A / RNA polymerase II Rabbit anti-Human Polyclonal (pSer5) Antibody
Application:
WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$287
LS-C750634-20
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
POLR2A / RNA polymerase II Rabbit anti-Human Polyclonal (pSer5) Antibody
Application:
WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
RNA polymerase II antibody LS-C750634 is an unconjugated rabbit polyclonal antibody to RNA polymerase II (POLR2A) (pSer5) from human. It is reactive with human and mouse. Validated for WB.
Target
Human POLR2A / RNA polymerase II
Synonyms
POLR2A | HRPB220 | HsRPB1 | POLR2 | RpIILS | RPOL2 | RNA Polymerase II | RPO2 | POLRA | RNA polymerase II subunit B1 | RPB1 | RPBh1
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Epitope
pSer5
Applications
  • Western blot (1:500 - 1:2000)
Usage
The predicted MW is 63kDa/217kDa, while the observed MW by Western blot was 260kDa.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About POLR2A / RNA polymerase II
P24928 NM_000937 NP_000928.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/16/2024