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Catalog Number Size Price
LS-C750568-20 20 µl $253 
LS-C750568-50 50 µl $306 
LS-C750568-100 100 µl $381 
LS-C750568-200 200 µl $489 
POLR2A / RNA polymerase II Antibody - Western blot analysis of extracts of 293T cells, using Phospho-POLR2A-S2 antibody at 1:1000 dilution. 293T cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37℃ for 1 hour or treated by Doxorubicin (0.5uM) for 24 hours. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. Blocking buffer: 3% BSA.An ECL Kit was used for detection and the exposure time was 1s.
POLR2A / RNA polymerase II Antibody - Immunoprecipitation analysis of 200ug extracts of MCF-7 cells, using 3 ug Phospho-POLR2A-S2 pAb. Western blot was performed from the immunoprecipitate using Phospho-POLR2A-S2 pAb at a dilition of 1:1000. MCF7 cells were treated by nocodazole (50 ng/ml) at 37°C for 20 hours.
POLR2A / RNA polymerase II Antibody - Western blot analysis of extracts of 293T cells, using Phospho-POLR2A-S2 antibody at 1:1000 dilution. 293T cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37℃ for 1 hour or treated by Doxorubicin (0.5uM) for 24 hours. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. Blocking buffer: 3% BSA.An ECL Kit was used for detection and the exposure time was 1s.
POLR2A / RNA polymerase II Antibody - Immunoprecipitation analysis of 200ug extracts of MCF-7 cells, using 3 ug Phospho-POLR2A-S2 pAb. Western blot was performed from the immunoprecipitate using Phospho-POLR2A-S2 pAb at a dilition of 1:1000. MCF7 cells were treated by nocodazole (50 ng/ml) at 37°C for 20 hours.
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Polyclonal Rabbit anti‑Human POLR2A / RNA polymerase II Antibody (phospho‑Ser2, WB) LS‑C750568

Polyclonal Rabbit anti‑Human POLR2A / RNA polymerase II Antibody (phospho‑Ser2, WB) LS‑C750568

Antibody:
POLR2A / RNA polymerase II Rabbit anti-Human Polyclonal (pSer2) Antibody
Application:
WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$253
LS-C750568-20
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
POLR2A / RNA polymerase II Rabbit anti-Human Polyclonal (pSer2) Antibody
Application:
WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
RNA polymerase II antibody LS-C750568 is an unconjugated rabbit polyclonal antibody to human RNA polymerase II (POLR2A) (pSer2). Validated for IP and WB.
Target
Human POLR2A / RNA polymerase II
Synonyms
POLR2A | HRPB220 | HsRPB1 | POLR2 | RpIILS | RPOL2 | RNA Polymerase II | RPO2 | POLRA | RNA polymerase II subunit B1 | RPB1 | RPBh1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Epitope
pSer2
Applications
  • Western blot (1:1000 - 1:2000)
  • Immunoprecipitation (1:50 - 1:100)
Usage
The predicted MW is 63kDa/217kDa, while the observed MW by Western blot was 260kDa.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About POLR2A / RNA polymerase II
P24928 NM_000937 NP_000928.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/14/2021