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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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POLE3 / DNA Polymerase Epsilon Antibody (clone 3C5.1) LS‑C2650
DNA Polymerase Epsilon antibody LS-C2650 is an unconjugated mouse monoclonal antibody to DNA Polymerase Epsilon (POLE3) from human, mouse, mustelid and other species. Validated for IP and WB.
Catalog
Size
Price
LS-C2650-50
50 µl (16 mg/ml)
$335
Description
DNA Polymerase Epsilon antibody LS-C2650 is an unconjugated mouse monoclonal antibody to DNA Polymerase Epsilon (POLE3) from human, mouse, mustelid and other species. Validated for IP and WB.
Target
Human POLE3 / DNA Polymerase Epsilon
Host
Mouse
Reactivity
Human, Mouse, Mustelid, Primate (tested or 100% immunogen sequence identity)
Non-Reactivity
Drosophila
Clonality
IgG2a Monoclonal [3C5.1]
Conjugations
Unconjugated. Also available conjugated with Biotin, DY650, DY488, DY550.
Purification
Protein G purified
Modifications
Unmodified
Applications
  • Western blot (5 µg/ml)
  • Immunoprecipitation
  • (applications tested for the base form of this product only)
Immunogen
Mono S FPLC column purified HeLa cell pol epsilon
Specificity
Human DNA pol epsilon. Does not recognize DNA pol alpha, beta or delta.
Presentation
Ascites.
Storage
Short term: -20°C; Long term: -70°C.
Restrictions
For research use only.
About POLE3 / DNA Polymerase Epsilon
Q9NRF9 NM_017443 NP_059139.3

Popular POLE3 / DNA Polymerase Epsilon Products

Detection of Human and Mouse PolE3/p17 by Immunohistochemistry. Sample: FFPE section of human ovarian carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-PolE3/p17 used at a dilution of 1:5000 (0.2 ug/ml). Detection: DAB.
Species: Human, Mouse, Rat, Bovine, Guinea pig
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation
Immunofluorescence analysis of U2OS cells.
Species: Human, Mouse
Applications: IHC, Immunofluorescence, Western blot
Antibody
Species: Human
Applications: IHC, Western blot, Immunoprecipitation, ELISA
Detection of Mouse PolE3/p17 by Immunohistochemistry. Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-PolE3/p17 used at a dilution of 1:100.
Species: Human, Mouse, Rat, Bovine, Guinea pig
Applications: IHC, IHC - Paraffin, Immunofluorescence
Immunofluorescent analysis of CHRAC17 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Species: Human, Mouse, Rat
Applications: ICC, Immunofluorescence, Western blot

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Images

Western blot

SDS-polyacrylamide gel analysis of HeLa DNA pol purified by Mono S FPLC. The Mono S fraction of HeLa DNA pol (0.1 mg) was electrophoresed on a 7% SDS-polyacrylamide gel and stained with silver.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
SDS-polyacrylamide gel analysis of HeLa DNA pol purified by Mono S FPLC. The Mono S fraction of HeLa DNA pol (0.1 mg) was electrophoresed on a 7% SDS-polyacrylamide gel and stained with silver. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

SDS-polyacrylamide gel analysis of HeLa DNA pol purified by Mono S FPLC. The Mono S fraction of HeLa DNA pol (0.1 mg) was electrophoresed on a 7% SDS-polyacrylamide gel and stained with silver.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
SDS-polyacrylamide gel analysis of HeLa DNA pol purified by Mono S FPLC. The Mono S fraction of HeLa DNA pol (0.1 mg) was electrophoresed on a 7% SDS-polyacrylamide gel and stained with silver. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

SDS-polyacrylamide gel analysis of HeLa DNA pol purified by Mono S FPLC. The Mono S fraction of HeLa DNA pol (0.1 mg) was electrophoresed on a 7% SDS-polyacrylamide gel and stained with silver.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
SDS-polyacrylamide gel analysis of HeLa DNA pol purified by Mono S FPLC. The Mono S fraction of HeLa DNA pol (0.1 mg) was electrophoresed on a 7% SDS-polyacrylamide gel and stained with silver. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

SDS-polyacrylamide gel analysis of HeLa DNA pol purified by Mono S FPLC. The Mono S fraction of HeLa DNA pol (0.1 mg) was electrophoresed on a 7% SDS-polyacrylamide gel and stained with silver.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
SDS-polyacrylamide gel analysis of HeLa DNA pol purified by Mono S FPLC. The Mono S fraction of HeLa DNA pol (0.1 mg) was electrophoresed on a 7% SDS-polyacrylamide gel and stained with silver. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 12/17/2018
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