Phospholamban antibody LS-C38229 is an unconjugated rabbit polyclonal antibody to Phospholamban (PLN) from human and bovine. Validated for ELISA and WB.
Human PLN / Phospholamban
(tested or 100% immunogen sequence identity)
Also available conjugated with AP
- Western blot (0.5 - 2 µg/ml)
- (applications tested for the base form of this product only)
Synthetic peptide corresponding to aa14-25 of human cardiac phospholamban with a cysteine added at the C-terminal (RA[pS]TIEMPQQAR-C) (KLH).
Recognizes human Phospholamban phosphorylated (Ser16) (Mr 5-25kD). Species cross-reactivity: bovine. Species sequence Homology: mouse, rat, rabbit, porcine and canine.
Suitable for use in ELISA and Western Blot. Proteins were visualized using a goat anti-rabbit IgG labeled with HRP and a chemiluminescence detection system. Note: Do not boil cardiac preparation. Boiling may reduce the 25kD pentamer in to tri-, di- or monomers. In addition, phosphorylation may disrupt the pentamer into dimers and monomers. Additional non-specific bands may be detected in some lysates (Mr 75 & 100kD).
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.
About PLN / Phospholamban