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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PLN / Phospholamban Antibody (aa2‑25) IHC‑plus™ LS‑B2358 Best Seller
Note: This antibody replaces LS-C26050
Phospholamban antibody LS-B2358 is an unconjugated mouse monoclonal antibody to Phospholamban (PLN) from human, bovine, dog and other species. Validated for ICC, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B2358-50
50 µl (1 mg/ml)
$485

Popular PLN / Phospholamban Products

Anti-PLN / Phospholamban antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.
Species: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Horse, Pig, Rabbit
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Antibody
Species: Human, Bovine
Applications: Western blot, ELISA
Antibody
Species: Human
Applications: IHC, Western blot, Flow Cytometry, ELISA
Antibody
Species: Human
Applications: ELISA, Dot Blot

Product Description

Phospholamban antibody LS-B2358 is an unconjugated mouse monoclonal antibody to Phospholamban (PLN) from human, bovine, dog and other species. Validated for ICC, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.

Specifications

Target
Dog PLN / Phospholamban
Synonyms
PLN, CMD1P, CMH18, PLB, Cardiac phospholamban, Phospholamban
Host
Mouse
Reactivity
Dog, Human, Bovine, Pig (tested or 100% immunogen sequence identity)
Predicted
Monkey, Mouse, Rat, Bat, Horse, Rabbit (at least 90% immunogen sequence identity)
Clonality
IgG2a Monoclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • ICC
  • Western blot
  • Immunoprecipitation
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic peptide corresponding to aa 2-25 (D2KVQYLTRSAIRRASTIEMPQQAR25) of dog PLB (phospholamban). Percent identity by BLAST analysis: Bovine, Dog, Pig (100%); Human, Monkey, Marmoset, Mouse, Rat, Panda, Bat, Horse, Rabbit, Opossum (96%); Gorilla, Gibbon (92%); Elephant (88%).
Epitope
aa2-25
Specificity
Recognizes canine PLB. Recognizes an epitope between aa 9-17 of canine PLB. Detects bands of ~25kD (non-reducing conditions; representing the pentameric form of PLB) and ~5kD (reducing conditions; representing the monomeric form of PLB) by Western Blot. Species cross-reactivity: human, mouse, rat, rabbit chicken, sheep, porcine and guinea pig.
Usage
Immunohistochemistry: LS-B2358 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2358 was determined to be 5 ug/ml. Western Blot: 2 ug/ml Detects bands of ~25kD (non-reducing conditions; representing the pentameric form of PLB) and ~5kD (reducing conditions; representing the monomeric form of PLB). Function: Increases the calcium uptake in ventricular SR vesicles.
Presentation
PBS, 0.05% Sodium Azide
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About PLN / Phospholamban
Reversibly inhibits the activity of ATP2A2 in cardiac sarcoplasmic reticulum by decreasing the apparent affinity of the ATPase for Ca2+. Modulates the contractility of the heart muscle in response to physiological stimuli via its effects on ATP2A2. Modulates calcium re-uptake during muscle relaxation and plays an important role in calcium homeostasis in the heart muscle. The degree of ATP2A2 inhibition depends on the oligomeric state of PLN. P26678 NM_002667 NP_002658.1


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Immunohistochemistry

Anti-PLN / Phospholamban antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-PLN / Phospholamban antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunohistochemistry

Anti-PLN / Phospholamban antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-PLN / Phospholamban antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunohistochemistry

Anti-PLN / Phospholamban antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-PLN / Phospholamban antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunohistochemistry

Anti-PLN / Phospholamban antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-PLN / Phospholamban antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Requested From: United States
Date Requested: 11/15/2018

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