PLK-1 antibody LS-C17320 is an unconjugated mouse monoclonal antibody to PLK-1 (PLK1) from human, mouse, rat and other species. Validated for ELISA, IF, IP and WB.
Human PLK1 / PLK-1
Human, Mouse, Rat, Xenopus
(tested or 100% immunogen sequence identity)
IgG1,k + IgG2b,k
Also available conjugated with AP
Protein A purified
- Immunofluorescence (2 - 5 µg/ml)
- Western blot (1 - 2 µg/ml)
- ELISA (0.1 - 1 µg/ml)
- (applications tested for the base form of this product only)
PLK1 / PLK-1 antibody was raised against a His-tagged Plk fusion protein (C-term Plk1), aa402-603 of the human Plk1 protein.
Recognizes the human ~68kD Plk1 protein. Cross-reactivity with a 110kD protein of unknown identity is sometimes observed. Species cross-reactivity: mouse, rat and Xenopus.
Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunoprecipitation. Immunofluorescence: 2-5 ug/ml. ELISA: 0.1-1 ug/ml. Western Blot: 1-2 ug/ml. Immunoprecipitation: 5 ug. Positive control: HeLa, A431 and NIH 3T3 cells.
PBS, pH 7.4, 0.1% Sodium Azide, 40% Glycerol
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.