Human PKG / PRKG1

Rabbit

Human, Mouse, Rat, Pig (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Protein A purified

Unmodified

A 15 residue synthetic peptide DEPPPDDNSGWDIDF based on the human PKG 1alpha (aa 657-671) and the peptide coupled to KLH. Percent identity by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Platypus (100%); Pufferfish (93%); Xenopus, Zebrafish (87%); Stickleback, Medaka, Seq squirt, Mosquito, Ant, Beetle (80%).

aa657-671

Recognizes human PKG1 alpha at ~75kD.

• IHC
• IHC - Paraffin (10 µg/ml)
• Western blot (1 µg/ml)

Immunohistochemistry: LS-B2219 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2219 was determined to be 10 ug/ml. Western Blot: Positive control: Mouse Brain Tissue Extract, Rat Brain Tissue Extract.

PBS, pH 7.2, 0.09% Sodium Azide, 50% Glycerol

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q13976 NCBI: NM_006258 NP_006249.1