PRKG1 antibody LS-B2219 is an unconjugated rabbit polyclonal antibody to PRKG1 (PKG) from human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Horse, Pig, Rabbit, Chicken
(tested or 100% immunogen sequence identity)
Protein A purified
- IHC - Paraffin (10 µg/ml)
- Western blot (1 µg/ml)
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PKG / PRKG1 antibody was raised against a 15 residue synthetic peptide DEPPPDDNSGWDIDF based on the human PKG 1alpha (residues 657-671) and the peptide coupled to KLH. Percent identity by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Platypus (100%); Pufferfish (93%); Xenopus, Zebrafish (87%); Stickleback, Medaka, Seq squirt, Mosquito, Ant, Beetle (80%).
Recognizes human PKG1 alpha at ~75kD.
Immunohistochemistry: LS-B2219 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2219 was determined to be 10 ug/ml. Western Blot: Positive control: Mouse Brain Tissue Extract, Rat Brain Tissue Extract.
PBS, pH 7.2, 0.09% Sodium Azide, 50% Glycerol
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.