Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Horse, Pig, Rabbit, Chicken
(tested or 100% immunogen sequence identity)
Protein A purified
- IHC - Paraffin (10 µg/ml)
- Western blot (1 µg/ml)
See our complete line of Immunohistochemistry Reagents
including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
PKG / PRKG1 antibody was raised against a 15 residue synthetic peptide DEPPPDDNSGWDIDF based on the human PKG 1alpha (residues 657-671) and the peptide coupled to KLH. Percent identity by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Platypus (100%); Pufferfish (93%); Xenopus, Zebrafish (87%); Stickleback, Medaka, Seq squirt, Mosquito, Ant, Beetle (80%).
Recognizes human PKG1 alpha at ~75kD.
Immunohistochemistry: LS-B2219 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2219 was determined to be 10 ug/ml. Western Blot: Positive control: Mouse Brain Tissue Extract, Rat Brain Tissue Extract.
PBS, pH 7.2, 50% glycerol, 0.09% azide.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.