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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PKG / PRKG1 Antibody (aa657‑671) IHC‑plus™ LS‑B2219
Note: This antibody replaces LS-C38517
PRKG1 antibody LS-B2219 is an unconjugated rabbit polyclonal antibody to PRKG1 (PKG) from human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Catalog
Size
Price
LS-B2219-50
50 µg (1 mg/ml)
Unavailable

Popular PKG / PRKG1 Products

Antibody
Species: Human
Applications: IHC, ICC, Western blot, Immunoprecipitation
Antibody
Species: Human, Mouse, Rat, Bovine
Applications: Western blot
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Species: Human, Mouse, Rat, Bovine
Applications: IHC, ICC, Western blot, Immunoprecipitation

Product Description

PRKG1 antibody LS-B2219 is an unconjugated rabbit polyclonal antibody to PRKG1 (PKG) from human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
About PKG / PRKG1
Serine/threonine protein kinase that acts as key mediator of the nitric oxide (NO)/cGMP signaling pathway. GMP binding activates PRKG1, which phosphorylates serines and threonines on many cellular proteins. Numerous protein targets for PRKG1 phosphorylation are implicated in modulating cellular calcium, but the contribution of each of these targets may vary substantially among cell types. Q13976 NM_006258 NP_006249.1

PRKG1 Antibody, CGKI Antibody, CGMP kinase I Antibody, CGMP protein kinase type I Antibody, CGK-I Antibody, CGK1 Antibody, PKGI Antibody, PKG I Antibody, PRKGR1B Antibody, PRKG1B Antibody, PRKGR1A Antibody, PGK Antibody, PKG Antibody, CGK Antibody, CGK 1 Antibody, CGKI-alpha Antibody, CGKI-BETA Antibody


Specifications

Target
Human PKG / PRKG1
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Horse, Pig, Rabbit, Chicken (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Western blot (1 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
PKG / PRKG1 antibody was raised against a 15 residue synthetic peptide DEPPPDDNSGWDIDF based on the human PKG 1alpha (residues 657-671) and the peptide coupled to KLH. Percent identity by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Platypus (100%); Pufferfish (93%); Xenopus, Zebrafish (87%); Stickleback, Medaka, Seq squirt, Mosquito, Ant, Beetle (80%).
Epitope
aa657-671
Specificity
Recognizes human PKG1 alpha at ~75kD.
Usage
Immunohistochemistry: LS-B2219 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2219 was determined to be 10 ug/ml. Western Blot: Positive control: Mouse Brain Tissue Extract, Rat Brain Tissue Extract.
Presentation
PBS, pH 7.2, 50% glycerol, 0.09% azide.
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

Publications (1)

1
Cyclic guanosine monophosphate and the dependent protein kinase regulate lymphatic contractility in rat thoracic duct. Gasheva OY, Gashev AA, Zawieja DC. The Journal of physiology. 2013 Sep;591:4549-65. [Full Text Article] [PubMed:23836689] [PMC:PMC3784198]

Customer Reviews (0)


Images


Immunohistochemistry

Anti-PRKG1 / CGKI antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2219 concentration 10 ug/ml.
Anti-PRKG1 / CGKI antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2219 concentration 10 ug/ml.

Immunohistochemistry

Anti-PRKG1 / CGKI antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2219 concentration 10 ug/ml.
Anti-PRKG1 / CGKI antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2219 concentration 10 ug/ml.

Immunohistochemistry

Anti-PRKG1 / CGKI antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2219 concentration 10 ug/ml.
Anti-PRKG1 / CGKI antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2219 concentration 10 ug/ml.

Immunohistochemistry

Anti-PRKG1 / CGKI antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2219 concentration 10 ug/ml.
Anti-PRKG1 / CGKI antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2219 concentration 10 ug/ml.


Requested From: 
Date Requested: 6/22/2018

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