PRKG1 antibody LS-C26172 is an unconjugated rabbit polyclonal antibody to human PRKG1 (PKG). Validated for ICC, IHC, IP and WB.
(tested or 100% immunogen sequence identity)
Monkey, Mouse, Bovine, Hamster, Chicken, Zebrafish (at least 90% immunogen sequence identity)
- IHC (1:400)
- Western blot (0.5 µg/ml)
- Immunoprecipitation (4 µg/ml)
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PKG / PRKG1 antibody was raised against a 14 residue synthetic peptide LRDLQWALQEKIEE based on the human PKG 1beta (residues 4-17), with a 1 amino acid substitution of a tryptophan residue for the tyrosine residue at position 9. A cysteine (C) residue was added and the peptide coupled to KLH. Percent identity by BLAST analysis: Human, Monkey, Mouse, Hamster, Panda, Bovine, Chicken, Stickleback, Medaka, Pufferfish, Zebrafish (93%).
Recognizes an ~80kD protein, corresponding to the apparent molecular mass of PKG 1beta. Species cross-reactivity: Human, mouse, rat and bovine.
Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Western Blot (ECL): 1:1000. Positive control: HeLa Cell Lysate and Rat Brain Tissue Extract.
PBS, 0.09% Sodium Azide, 50% Glycerol
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.