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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PKG / PRKG1 Antibody (aa4‑17) LS‑C26172
PRKG1 antibody LS-C26172 is an unconjugated rabbit polyclonal antibody to human PRKG1 (PKG). Validated for ICC, IHC, IP and WB.
Catalog
Size
Price
LS-C26172-50
50 µg (1 mg/ml)
$405
LS-C26172-200
200 µg
$530
Description
PRKG1 antibody LS-C26172 is an unconjugated rabbit polyclonal antibody to human PRKG1 (PKG). Validated for ICC, IHC, IP and WB.
Target
Human PKG / PRKG1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Monkey, Mouse, Bovine, Hamster, Chicken, Zebrafish (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC (1:400)
  • ICC
  • Western blot (0.5 µg/ml)
  • Immunoprecipitation (4 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
PKG / PRKG1 antibody was raised against a 14 residue synthetic peptide LRDLQWALQEKIEE based on the human PKG 1beta (residues 4-17), with a 1 amino acid substitution of a tryptophan residue for the tyrosine residue at position 9. A cysteine (C) residue was added and the peptide coupled to KLH. Percent identity by BLAST analysis: Human, Monkey, Mouse, Hamster, Panda, Bovine, Chicken, Stickleback, Medaka, Pufferfish, Zebrafish (93%).
Epitope
aa4-17
Specificity
Recognizes an ~80kD protein, corresponding to the apparent molecular mass of PKG 1beta. Species cross-reactivity: Human, mouse, rat and bovine.
Usage
Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Western Blot (ECL): 1:1000. Positive control: HeLa Cell Lysate and Rat Brain Tissue Extract.
Presentation
PBS, 0.09% Sodium Azide, 50% Glycerol
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About PKG / PRKG1
Q13976 NM_006258 NP_006249.1

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Requested From: United States
Date Requested: 12/18/2018
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