PIP5K1C | Diphosphoinositide kinase | KIAA0589 | LCCS3 | PIP5K1-gamma | Type I PIP kinase | PIP5KIgamma | PtdIns(4)P-5-kinase 1 gamma | PIP5K-GAMMA | PIP5Kgamma | PIPKIg_v4
(tested or 100% immunogen sequence identity)
Synthetic peptide from human PIP5K1C.
Binds to a 85-90kD human protein. Binding can be blocked by the peptide immunogen.
- Western blot
- ELISA (1:50000)
Western Blot. ELISA: 1:50000. ELISA: Antibody titers and specificity were analyzed by ELISA assay using control samples (varying concentration of GST-EPOr) and control anti GST antibody. Affinity purified antibody was found to detect EPOr at concentrations of 50 ng/ml. Neutralization of ligand binding has been detected using Erythroleukemia cell line TF-1 (3000 EPO receptors/cell) to block Iodine-labeled erythropoietin ligand binding. Western blot concentration used 10 ug/ml. It will recognize EPOr-GST at its migration of 49kD on SDS-PAGE. Good secondary anti-sheep reagents are essential for this assay. Recommended use of rabbit anti-sheep rather than donkey anti-sheep antibodies as a secondary labeling reagents.
PBS, pH 7.2, 1 mg/ml BSA, 0.1% Sodium Azide, 40% Glycerol
For research use only. Intended for use by laboratory professionals.
This antibody carries the LSBio 100% Guarantee.