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Antibodies > PIK3R2 / p85 Beta > LS-C312832
 
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Catalog Number Size Price
LS-C312832-10 10 µg $318 
LS-C312832-100 100 µg (0.5 mg/ml) $470 
PIK3R2 / p85 Beta Antibody - PIK3R2 / p85 Beta antibody. IHC(P): Human Breast Cancer Tissue.
PIK3R2 / p85 Beta Antibody - Western blot analysis of PIK3R2 using anti-PIK3R2 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse liver tissue lysates, Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIK3R2 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PIK3R2 at approximately 85KD. The expected band size for PIK3R2 is at 85KD.
PIK3R2 / p85 Beta Antibody - Western blot analysis of PIK3R2 using anti-PIK3R2 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Testis tissue lysates, Lane 2: 293T whole cell lysates, Lane 3: HELA whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIK3R2 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PIK3R2 at approximately 85KD. The expected band size for PIK3R2 is at 85KD.
PIK3R2 / p85 Beta Antibody - WB of PIK3R2 / p85 Beta antibody. Lane 1: Rat Testis Tissue Lysate. Lane 2: 293T Cell Lysate. Lane 3: HELA Cell Lysate.
PIK3R2 / p85 Beta Antibody - PIK3R2 / p85 Beta antibody. IHC(P): Human Breast Cancer Tissue.
PIK3R2 / p85 Beta Antibody - Western blot analysis of PIK3R2 using anti-PIK3R2 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse liver tissue lysates, Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIK3R2 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PIK3R2 at approximately 85KD. The expected band size for PIK3R2 is at 85KD.
PIK3R2 / p85 Beta Antibody - Western blot analysis of PIK3R2 using anti-PIK3R2 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Testis tissue lysates, Lane 2: 293T whole cell lysates, Lane 3: HELA whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIK3R2 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PIK3R2 at approximately 85KD. The expected band size for PIK3R2 is at 85KD.
PIK3R2 / p85 Beta Antibody - WB of PIK3R2 / p85 Beta antibody. Lane 1: Rat Testis Tissue Lysate. Lane 2: 293T Cell Lysate. Lane 3: HELA Cell Lysate.
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Polyclonal Rabbit anti‑Human PIK3R2 / p85 Beta Antibody (aa447‑461, IHC, WB) LS‑C312832

Polyclonal Rabbit anti‑Human PIK3R2 / p85 Beta Antibody (aa447‑461, IHC, WB) LS‑C312832

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Note: This antibody replaces LS-C389067
Antibody:
PIK3R2 / p85 Beta Rabbit anti-Human Polyclonal (aa447-461) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Monkey, Mouse, Rat, Bat, Bovine, Guinea pig, Pig, Sheep
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C312832-10
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
PIK3R2 / p85 Beta Rabbit anti-Human Polyclonal (aa447-461) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Monkey, Mouse, Rat, Bat, Bovine, Guinea pig, Pig, Sheep
Format:
Unconjugated, Unmodified

Specifications

Description
P85 Beta antibody LS-C312832 is an unconjugated rabbit polyclonal antibody to p85 Beta (PIK3R2) (aa447-461) from human. It is reactive with human, mouse, rat and other species. Validated for IHC and WB.
Target
Human PIK3R2 / p85 Beta
Synonyms
PIK3R2 | p85 | p85B | p85-BETA | PI3K regulatory subunit beta | MPPH | PI3-kinase subunit p85-beta
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Guinea pig, Pig, Sheep (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human PIK3R2 (447-461 aa KVYHQQYQDKSREYD), identical to the related rat and mouse sequences.
Epitope
aa447-461
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg Thimerosal, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PIK3R2 / p85 Beta
O00459 NM_005027 NP_005018.1

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Species: Human
Applications: IHC, IHC - Paraffin, Western blot
PIK3R2 / p85 Beta Antibody - Formalin-fixed and paraffin-embedded human brain tissue reacted with PIK3R2 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Species: Human
Applications: IHC, IHC - Paraffin, Western blot
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See all PIK3R2 / p85 Beta Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/25/2024
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