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Catalog Number Size Price
LS-B13741-200 200 µl $460 
PIK3C3 / VPS34 Antibody - Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
PIK3C3 / VPS34 Antibody - Fluorescent image of U251 cells stained with PI3KC3 (S34) antibody. U251 cells were treated with Chloroquine (50 mu M,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated PI3KC3 (S34) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). PI3KC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
PIK3C3 / VPS34 Antibody - Western blot of PI3KC3 (S34) in HeLa cell line lysates (35 ug/lane). PI3KC3 (arrow) was detected using the purified antibody.
PIK3C3 / VPS34 Antibody - Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
PIK3C3 / VPS34 Antibody - Fluorescent image of U251 cells stained with PI3KC3 (S34) antibody. U251 cells were treated with Chloroquine (50 mu M,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated PI3KC3 (S34) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). PI3KC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
PIK3C3 / VPS34 Antibody - Western blot of PI3KC3 (S34) in HeLa cell line lysates (35 ug/lane). PI3KC3 (arrow) was detected using the purified antibody.
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IHC‑plus™ Polyclonal Rabbit anti‑Human PIK3C3 / VPS34 Antibody (Ser34, IHC, IF, WB) LS‑B13741

IHC‑plus™ Polyclonal Rabbit anti‑Human PIK3C3 / VPS34 Antibody (Ser34, IHC, IF, WB) LS‑B13741

Note: This antibody replaces LS-C163705
Antibody:
PIK3C3 / VPS34 Rabbit anti-Human Polyclonal (Ser34) Antibody
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B13741-200
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
PIK3C3 / VPS34 Rabbit anti-Human Polyclonal (Ser34) Antibody
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
VPS34 antibody LS-B13741 is an unconjugated rabbit polyclonal antibody to human VPS34 (PIK3C3) (Ser34). Validated for IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human PIK3C3 / VPS34
Synonyms
PIK3C3 | HVps34 | Phosphatidylinositol 3-kinase | VPS34 | PI3-kinase type 3 | PI3K type 3 | PtdIns-3-kinase type 3
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat, Pig, Xenopus (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Epitope
Ser34
Specificity
This PI3KC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 14-39 amino acids from human PI3KC3.
Applications
  • IHC
  • IHC - Paraffin (1:10 - 1:100)
  • Immunofluorescence (1:200)
  • Western blot (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PIK3C3 / VPS34
Q8NEB9 NM_002647 NP_002638.2

Validation

PIK3C3 / VPS34 Antibody - Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)

LSBio Ratings

IHC-plus™ PIK3C3 / VPS34 Antibody (Ser34) for IHC, IF/Immunofluorescence, WB/Western LS-B13741 has an LSBio Rating of
Laboratory Validation Score (4)

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/25/2024