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Catalog Number Size Price
LS-C347210-2 2 ml $481 
Mouse IgG Antibody - Mouse IgG was used in a 1:25 dilution on swine colon, in combination with the primary monoclonal antibody to vimentin, RV202 (Vimentin), used in a 1:1000 dilution. The green staining shows the connective tissue cells, while no staining is seen in the epithelial cells of the intestinal crypts.

Polyclonal Pig Anti‑Mouse IgG Secondary Antibody (FITC, IHC, IF) LS‑C347210

Polyclonal Pig Anti‑Mouse IgG Secondary Antibody (FITC, IHC, IF) LS‑C347210

Antibody:
Mouse IgG Pig anti-Mouse Polyclonal (FITC) Antibody
Application:
IHC, IHC-Fr, ICC, IF, ELISA, ID
Reactivity:
Mouse
Format:
FITC, Unmodified
Other formats:
Price
Catalog Number
$481
LS-C347210-2
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
Mouse IgG Pig anti-Mouse Polyclonal (FITC) Antibody
Application:
IHC, IHC-Fr, ICC, IF, ELISA, ID
Reactivity:
Mouse
Format:
FITC, Unmodified
Other formats:

Specifications

Description
Mouse IgG antibody LS-C347210 is an FITC-conjugated pig polyclonal antibody. Validated for ELISA, ICC, ID, IF and IHC.
Host
Pig
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
FITC. Also available Unconjugated.
Purification
Purified IgG
Modifications
Unmodified
Immunogen
Purified normal IgG isolated from pooled mouse serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
Specificity
Mouse IgG, heavy and light chains.
Applications
  • IHC
  • IHC - Frozen
  • ICC
  • Immunofluorescence
  • ELISA
  • Immunodiffusion
  • Applications tested for the base form of this product only
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgG molecule. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion. It does not react with any non-Ig protein in mouse serum, as tested by immunoelectrophoresis and double radial immunodiffusion. To identify and measure IgG, antigen or antibody, at the cellular and subcellular level by immunofluorescence direct staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases. to identify a specific antigen or immune complex using a reference antibody of mouse origin in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labeled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:20 and 1:80.
Presentation
Lyophilized from PBS, pH 7.2, No preservatives added
Reconstitution
2 ml Distilled water
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/15/2024