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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Seattle WA 98121

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Phosphotyrosine Antibody (AP)

Phosphotyrosine antibody LS-C510615 is an AP-conjugated mouse monoclonal antibody to Phosphotyrosine. Validated for IP and WB.
Catalog
Size
Price
LS-C510615-100
100 µl
$755
Description
Phosphotyrosine antibody LS-C510615 is an AP-conjugated mouse monoclonal antibody to Phosphotyrosine. Validated for IP and WB.
Target
Phosphotyrosine
Host
Mouse
Clonality
IgG1,k Monoclonal
Conjugations
Alkaline Phosphatase. Also available Unconjugated or conjugated with APC, Biotin, FITC, HRP, PE.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot
  • Immunoprecipitation
  • (applications tested for the base form of this product only)
Immunogen
Phosphotyrosine antibody was raised against tyrosine-phosphorylated proteins.
Specificity
PY-Plus reacts specifically with tyrosine-phosphorylated proteins. It shows no cross-reactivity to phosphoserine or phosphothreonine. On Western blots, specific inhibition of each individual antibody and the cocktail reactivity can be achieved by pre-incubation of the antibody with 20 mM phosphotyrosine, whereas pre-incubation with 20 mM phosphoserine or 20 mM phosphothreonine is ineffective. Species cross-reactivity: It is well established that no one monoclonal antibody can strongly react with all tyrosine-phosphorylated proteins. PY-Plus contains 2 different monoclonal antibodies that are designed to strongly react with the broadest range of tyrosine phosphorylated proteins possible. It will react with tyrosine-phosphorylated proteins in both the native and denatured states. Positive reactivity is independent of the species source and has been observed in the following samples: mouse brain, NIH 3T3 cells (+/-TPA), K562 cells, EGF-stimulated A431 cells and MCF-7 cells.
Presentation
PBS, pH 7.2, No preservatives added
Storage
Store at 4°C.
Restrictions
For research use only.

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Requested From: United States
Date Requested: 1/21/2019
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