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Phosphothreonine Antibody LS‑C74909

Catalog Number Size Price
LS-C74909-100 100 µg $635 
Antibody

Phosphothreonine Antibody LS‑C74909

Antibody:
Mouse Monoclonal to Phosphothreonine
Application:
WB, IP, ELISA
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$635 
LS-C74909-100
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Monoclonal to Phosphothreonine
Application:
WB, IP, ELISA
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
Phosphothreonine antibody LS-C74909 is an unconjugated mouse monoclonal antibody to Phosphothreonine. Validated for ELISA, IP and WB.
Target
Phosphothreonine
Host
Mouse
Clonality
IgG2a,k Monoclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (2 µg/ml)
  • Immunoprecipitation
  • ELISA (0.5 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
Keyhole Limpet Hemocyanin (KLH)- conjugated phosphothreonine conjugates and Phosvitin.
Specificity
Recognizes Phosphothreonine, peptidyl-phosphothreonine, and threonine-phosphorylated proteins. Binding of Ly-pT-Gly-HRP (0.2 ug/100 ul/well) to an anti-phosphothreonine coated microplate (0.5 ug/100 ul/well) is inhibited by 95% with the addition of 2-10 ug/ml anti-phosphothreonine. No inhibition is evident by inhibition of phosphoserine, phosphotyrosine or threonine at concentrations of up to 1 mg/ml. Species cross-reactivity: species independent.
Usage
Suitable for use in ELISA, Western Blot, and Immunoprecipitation. ELISA: 0.5 ug/ml (kinase assay). Western Blot: 2 ug/ml versus mouse brain lysate. Incubate at RT for 4 hours.
Presentation
PBS, pH 7.4, 0.1% Sodium Azide
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Requested From: United States
Date Requested: 9/23/2019