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Phosphothreonine Antibody (clone 18F6) LS‑C60053

Catalog Number Size Price
LS-C60053-100 100 µl $425 
Anti-Phosphothreonine Antibody - Western Blot. Mab anti-phosphothreonine antibody (clone 18F6) is shown to detect threonine phosphorylation of proteins in a lysate from A431 cells stimulated with EGF. Separation is achieved under reducing conditions using a pre-cast 4-20% iGel from Gradipore, Inc. A 1:1000 dilution of Mab anti-phosphothreonine is used for 30 min followed by detection using a 1:2500 dilution of IRDye 800 conjugated Goat-a-Mouse IgG [H&L] ( and visualization using the Odyssey Infrared Imaging System developed by LI-COR. Other detection systems will yield similar results. IRDye is a trademark of LI-COR, Inc.
Anti-Phosphothreonine Antibody - ELISA. ELISA results of Mab anti-phosphothreonine antibody tested against BSA conjugates of pT, pY and pS. Each well was coated with 0.1 ug of conjugate. The starting dilution of antibody was 1:1000 and each point on the X-axis represents a 2-fold dilution. HRP conjugated Gt-a-Mouse IgG (H&L) and TMB substrate were used for detection.
Anti-Phosphothreonine Antibody - Western Blot. Mab anti-phosphothreonine antibody (clone 18F6) is shown to detect threonine phosphorylation of proteins in a lysate from A431 cells stimulated with EGF. Separation is achieved under reducing conditions using a pre-cast 4-20% iGel from Gradipore, Inc. A 1:1000 dilution of Mab anti-phosphothreonine is used for 30 min followed by detection using a 1:2500 dilution of IRDye 800 conjugated Goat-a-Mouse IgG [H&L] ( and visualization using the Odyssey Infrared Imaging System developed by LI-COR. Other detection systems will yield similar results. IRDye is a trademark of LI-COR, Inc.
Anti-Phosphothreonine Antibody - ELISA. ELISA results of Mab anti-phosphothreonine antibody tested against BSA conjugates of pT, pY and pS. Each well was coated with 0.1 ug of conjugate. The starting dilution of antibody was 1:1000 and each point on the X-axis represents a 2-fold dilution. HRP conjugated Gt-a-Mouse IgG (H&L) and TMB substrate were used for detection.
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Phosphothreonine Antibody (clone 18F6) LS‑C60053

Antibody:
Mouse Monoclonal to Phosphothreonine
Application:
WB, ELISA
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-C60053-100
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Monoclonal to Phosphothreonine
Application:
WB, ELISA
Format:
Unconjugated, Unmodified

Specifications

Description
Phosphothreonine antibody LS-C60053 is an unconjugated mouse monoclonal antibody to Phosphothreonine. Validated for ELISA and WB.
Target
Phosphothreonine
Host
Mouse
Clonality
Monoclonal
Clone
18F6
Conjugations
Unconjugated
Purification
Ascites
Modifications
Unmodified
Applications
  • Western blot
  • ELISA
Immunogen
Phosphothreonine conjugated KLH.
Specificity
Reactivity is specific for phosphothreonine and minimal cross reactivity is observed against phosphoserine or phosphotyrosine
Usage
This monoclonal antibody reacts specifically with phosphothreonine and shows minimal reactivity by ELISA, and competitive ELISA, with phosphoserine or phosphotyrosine. The antibody reacts with free phosphothreonine, phosphothreonine conjugated to carriers such as thyroglobulin or BSA, as well as phosphothreonine in proteins of both unstimulated and stimulated cell lysates. Although not tested, this antibody is likely functional in RIA, flow cytometry, immunohistochemistry and immunoprecipitation. Phosphorylation of threonine residues is associated with many growth factors and oncogene protein kinases and is important for cell signaling in activation, proliferation and differentiation.
Presentation
0.01% Sodium Azide
Storage
Store at -20°C. Aliquot to avoid freeze-thaw cycles. Store undiluted.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Reactivity: Rat
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Requested From: United States
Date Requested: 9/21/2019