(tested or 100% immunogen sequence identity)
Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Alkaline Phosphatase [E.coli]
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Alkaline Phosphatase [E.coli]. Cross reactivity against Alkaline Phosphatase from other tissues and species may occur but have not been specifically determined. Some minimal crossreactivity has been reported when lysates of cell extracts overproducing E.coli proteins are probed.
Western blot (1:500 - 1:2000)
ELISA (1:4000 - 1:20000)
Applications should be user optimized.
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
For research use only. Intended for use by laboratory professionals.