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Catalog Number	Size	Price
LS-C745057-25	25 µl (1 mg/ml)	$304

PHOA Antibody - Western Blot of rabbit anti-Alkaline Phosphatase (E. Coli) Antibody. Lane 1: Alkaline Phosphatase (E. Coli). Load: 50 ng per lane. Primary antibody: Rabbit anti-Alkaline Phosphatase (E. Coli) Antibody at 1:1,000 overnight at 4°C. Secondary antibody: Peroxidase Rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 50 kDa, 50 kDa for Alkaline Phosphatase (E. Coli).

Polyclonal Rabbit anti‑E. coli PHOA Antibody (Liquid, WB) LS‑C745057

Name: Anti-PHOA Antibody | Rabbit anti-E. coli Polyclonal Liquid ELISA,WB | LSBio
Brand: Lifespan BioSciences
SKU: LS-C745057-25
Price: 304.0000 USD
Availability: InStock

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Antibody:

PHOA Rabbit anti-E. coli Polyclonal (Liquid) Antibody

Application:

WB, ELISA

Reactivity:

E. coli

Format:

Unconjugated, Liquid

$304

LS-C745057-25

Toll Free North America

206-374-1102

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

PHOA Rabbit anti-E. coli Polyclonal (Liquid) Antibody

Application:

WB, ELISA

Reactivity:

E. coli

Format:

Unconjugated, Liquid

Specifications

Description

PHOA antibody LS-C745057 is an unconjugated rabbit polyclonal antibody to e. coli PHOA. Validated for ELISA and WB.

Target

E. coli PHOA

Synonyms

PHOA | Bacterial alkaline phosphatase | JW0374 | PsiA | ECK0378

See All PHOA Antibodies

Host

Rabbit

Reactivity

E. coli (tested or 100% immunogen sequence identity)

Clonality

IgG Polyclonal

Conjugations

Unconjugated

Purification

Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.

Modifications

Liquid

Immunogen

Alkaline Phosphatase [E.coli]

Specificity

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Alkaline Phosphatase [E.coli]. Cross reactivity against Alkaline Phosphatase from other tissues and species may occur but have not been specifically determined. Some minimal crossreactivity has been reported when lysates of cell extracts overproducing E.coli proteins are probed.

Applications

Western blot (1:500 - 1:2000)
ELISA (1:4000 - 1:20000)

Usage

Applications should be user optimized.

Presentation

0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Storage

Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About PHOA

Uniprot: P00634 NCBI: P00634