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Catalog Number Size Price
LS-C745057-25 25 µl (1 mg/ml) $275 
PHOA Antibody - Western Blot of rabbit anti-Alkaline Phosphatase (E. Coli) Antibody. Lane 1: Alkaline Phosphatase (E. Coli). Load: 50 ng per lane. Primary antibody: Rabbit anti-Alkaline Phosphatase (E. Coli) Antibody at 1:1,000 overnight at 4°C. Secondary antibody: Peroxidase Rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 50 kDa, 50 kDa for Alkaline Phosphatase (E. Coli).

Polyclonal Rabbit anti‑E. coli PHOA Antibody (Liquid, WB) LS‑C745057

Polyclonal Rabbit anti‑E. coli PHOA Antibody (Liquid, WB) LS‑C745057

Antibody:
PHOA Rabbit anti-E. coli Polyclonal (Liquid) Antibody
Application:
WB, ELISA
Reactivity:
E. coli
Format:
Unconjugated, Liquid
Price
Catalog Number
$275
LS-C745057-25
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
PHOA Rabbit anti-E. coli Polyclonal (Liquid) Antibody
Application:
WB, ELISA
Reactivity:
E. coli
Format:
Unconjugated, Liquid

Specifications

Description
PHOA antibody LS-C745057 is an unconjugated rabbit polyclonal antibody to e. coli PHOA. Validated for ELISA and WB.
Target
E. coli PHOA
Synonyms
PHOA | Bacterial alkaline phosphatase | JW0374 | PsiA | ECK0378
Host
Rabbit
Reactivity
E. coli (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Modifications
Liquid
Immunogen
Alkaline Phosphatase [E.coli]
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Alkaline Phosphatase [E.coli]. Cross reactivity against Alkaline Phosphatase from other tissues and species may occur but have not been specifically determined. Some minimal crossreactivity has been reported when lysates of cell extracts overproducing E.coli proteins are probed.
Applications
  • Western blot (1:500 - 1:2000)
  • ELISA (1:4000 - 1:20000)
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PHOA
P00634 P00634

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/17/2021