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Antibodies > PGK1 / Phosphoglycerate Kinase > LS-C749054
 
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Catalog Number Size Price
LS-C749054-20 20 µl $275 
LS-C749054-50 50 µl $311 
LS-C749054-100 100 µl $389 
LS-C749054-200 200 µl $503 
PGK1 / Phosphoglycerate Kinase Antibody - Immunohistochemistry of paraffin-embedded human esophagus using PGK1 Antibody at dilution of 1:100 (40x lens).
PGK1 / Phosphoglycerate Kinase Antibody - Immunohistochemistry of paraffin-embedded mouse kidney using PGK1 Antibody at dilution of 1:100 (40x lens).
PGK1 / Phosphoglycerate Kinase Antibody - Immunohistochemistry of paraffin-embedded rat intestine using PGK1 Antibody at dilution of 1:100 (40x lens).
PGK1 / Phosphoglycerate Kinase Antibody - Immunohistochemistry of paraffin-embedded human colon using PGK1 Antibody at dilution of 1:100 (40x lens).
PGK1 / Phosphoglycerate Kinase Antibody - Western blot analysis of extracts of various cell lines, using PGK1 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
PGK1 / Phosphoglycerate Kinase Antibody - Immunohistochemistry of paraffin-embedded human esophagus using PGK1 Antibody at dilution of 1:100 (40x lens).
PGK1 / Phosphoglycerate Kinase Antibody - Immunohistochemistry of paraffin-embedded mouse kidney using PGK1 Antibody at dilution of 1:100 (40x lens).
PGK1 / Phosphoglycerate Kinase Antibody - Immunohistochemistry of paraffin-embedded rat intestine using PGK1 Antibody at dilution of 1:100 (40x lens).
PGK1 / Phosphoglycerate Kinase Antibody - Immunohistochemistry of paraffin-embedded human colon using PGK1 Antibody at dilution of 1:100 (40x lens).
PGK1 / Phosphoglycerate Kinase Antibody - Western blot analysis of extracts of various cell lines, using PGK1 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
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Polyclonal Rabbit anti‑Human PGK1 / Phosphoglycerate Kinase Antibody (IHC, WB) LS‑C749054

Polyclonal Rabbit anti‑Human PGK1 / Phosphoglycerate Kinase Antibody (IHC, WB) LS‑C749054

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Antibody:
PGK1 / Phosphoglycerate Kinase Rabbit anti-Human Polyclonal Antibody
Application:
IHC, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$275
LS-C749054-20
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
PGK1 / Phosphoglycerate Kinase Rabbit anti-Human Polyclonal Antibody
Application:
IHC, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
Phosphoglycerate Kinase antibody LS-C749054 is an unconjugated rabbit polyclonal antibody to Phosphoglycerate Kinase (PGK1) from human. It is reactive with human, mouse and rat. Validated for IHC and WB.
Target
Human PGK1 / Phosphoglycerate Kinase
Synonyms
PGK1 | MIG10 | Primer recognition protein 2 | PRP 2 | PGKA | Phosphoglycerate kinase 1
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-417 of human PGK1 (NP_000282.1). MSLSNKLTLDKLDVKGKRVVMRVDFNVPMKNNQITNNQRIKAAVPSIKFCLDNGAKSVVLMSHLGRPDGVPMPDKYSLEPVAVELKSLLGKDVLFLKDCVGPEVEKACANPAAGSVILLENLRFHVEEEGKGKDASGNKVKAEPAKIEAFRASLSKLGDVYVNDAFGTAHRAHSSMVGVNLPQKAGGFLMKKELNYFAKALESPERPFLAILGGAKVADKIQLINNMLDKVNEMIIGGGMAFTFLKVLNNMEIGTSLFDEEGAKIVKDLMSKAEKNGVKITLPVDFVTADKFDENAKTGQATVASGIPAGWMGLDCGPESSKKYAEAVTRAKQIVWNGPVGVFEWEAFARGTKALMDEVVKATSRGCITIIGGGDTATCCAKWNTED
Applications
  • IHC (1:50 - 1:200)
  • Western blot (1:500 - 1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
The predicted MW is 41kDa/44kDa, while the observed MW by Western blot was 47kDa.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PGK1 / Phosphoglycerate Kinase
P00558 NM_000291 NP_000282.1

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Featured Products

PGK1 / Phosphoglycerate Kinase Antibody - Anti-PGK1 antibody IHC of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
PGK1 / Phosphoglycerate Kinase Antibody (aa102‑151) LS‑B5809
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Western blot
PGK1 / Phosphoglycerate Kinase Antibody - PGK1 antibody PGK1(phosphoglycerate kinase 1) Antibody was used in IHC to stain formalin-fixed, paraffin-embedded human intestine. This image was taken for the unconjugated form of this product. Other forms have not been tested.
PGK1 / Phosphoglycerate Kinase Antibody (aa301‑350) LS‑C81163
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
PGK1 / Phosphoglycerate Kinase Antibody - Anti-PGK1/Phosphoglycerate Kinase 1 antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
PGK1 / Phosphoglycerate Kinase Antibody (Biotin) LS‑C211232
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Western blot, ELISA
PGK1 / Phosphoglycerate Kinase Antibody - Knockout Varification: Lane 1: Wild-type Hela cell lysate; Lane 2: PGK1 knockout Hela cell lysate; Predicted MW: 45kDa ; Observed MW: 45kDa; Primary Ab: 1µg/ml Rabbit Anti-Human PGK1 Antibody; Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;
PGK1 / Phosphoglycerate Kinase Antibody (aa2‑417) LS‑C373462
Species: Human, Pig
Applications: ICC, Western blot, Immunoprecipitation
PGK1 / Phosphoglycerate Kinase Antibody - Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PGK1 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
PGK1 / Phosphoglycerate Kinase Antibody (aa117‑145) LS‑C98055
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot, Flow Cytometry
PGK1 / Phosphoglycerate Kinase Antibody - Immunofluorescent analysis of PGK1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
PGK1 / Phosphoglycerate Kinase Antibody LS‑C482333
Species: Human, Mouse, Rat
Applications: ICC, Immunofluorescence, Western blot
See all PGK1 / Phosphoglycerate Kinase Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/18/2024
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