Target
Human PGK1 / Phosphoglycerate Kinase
Synonyms
PGK1 | MIG10 | Primer recognition protein 2 | PRP 2 | PGKA | Phosphoglycerate kinase 1
Reactivity
Human, Monkey
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence C-SKKYAEAVTRAKQ, from the internal region of the protein sequence according to NP_000282.1.
Specificity
Human PGK1/Phosphoglycerate Kinase 1.
Applications
- Western blot (0.1 - 0.3 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:128000)
Usage
Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 45kD band observed in lysates of cell lines A432, HEK293, HeLa and HepG2 (calculated MW of 44.6kD according to NP_000282.1). Recommended concentration: 0.1-0.3 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PGK1 / Phosphoglycerate Kinase