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Catalog Number Size Price
LS-C744792-25 25 µl (1 mg/ml) $275 
PFKM / PFK-1 Antibody - #Confocal microscopy images using the anti-F6PK MAGP-2 antibody shows specific staining.  Cultures of A7r5 VSM cells (rat aorta) were labeled with anti-F6PK (PFK) at 1:200 and anti-caveolin (CAV)-1.  Images from labeled A7r5 cells were acquired separately for PFK fluorescence (green) and CAV-1 fluorescence (red) from each cell.
PFKM / PFK-1 Antibody - Western blot analysis demonstrates the labeling specificity of the antibodies used for immunofluorescence (see Fig. 1). Tissue lysate from hog carotid arteries was analyzed by SDS-PAGE. Nitrocellulose membranes were probed with the corresponding antibodies at the same concentrations used for immunofluorescence labeling.  0.05 (PFK) and 0.2 mg (CAV-1) was loaded into lanes 1 and 2, respectively. Western blot development resulted in identification of the proteins of interest at the expected sizes, demonstrating the labeling specificity of the antibodies. Molecular weights are given.
PFKM / PFK-1 Antibody - #Confocal microscopy images using the anti-F6PK MAGP-2 antibody shows specific staining.  Cultures of A7r5 VSM cells (rat aorta) were labeled with anti-F6PK (PFK) at 1:200 and anti-caveolin (CAV)-1.  Images from labeled A7r5 cells were acquired separately for PFK fluorescence (green) and CAV-1 fluorescence (red) from each cell.
PFKM / PFK-1 Antibody - Western blot analysis demonstrates the labeling specificity of the antibodies used for immunofluorescence (see Fig. 1). Tissue lysate from hog carotid arteries was analyzed by SDS-PAGE. Nitrocellulose membranes were probed with the corresponding antibodies at the same concentrations used for immunofluorescence labeling.  0.05 (PFK) and 0.2 mg (CAV-1) was loaded into lanes 1 and 2, respectively. Western blot development resulted in identification of the proteins of interest at the expected sizes, demonstrating the labeling specificity of the antibodies. Molecular weights are given.
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Polyclonal Goat anti‑Rabbit PFKM / PFK‑1 Antibody (Liquid, IF, WB) LS‑C744792

Polyclonal Goat anti‑Rabbit PFKM / PFK‑1 Antibody (Liquid, IF, WB) LS‑C744792

Antibody:
PFKM / PFK-1 Goat anti-Rabbit Polyclonal (Liquid) Antibody
Application:
IF, WB, ELISA
Reactivity:
Rabbit
Format:
Unconjugated, Liquid
Price
Catalog Number
$275
LS-C744792-25
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
PFKM / PFK-1 Goat anti-Rabbit Polyclonal (Liquid) Antibody
Application:
IF, WB, ELISA
Reactivity:
Rabbit
Format:
Unconjugated, Liquid

Specifications

Description
PFK-1 antibody LS-C744792 is an unconjugated goat polyclonal antibody to rabbit PFK-1 (PFKM). Validated for ELISA, IF and WB.
Target
Rabbit PFKM / PFK-1
Synonyms
PFKM | 6-phosphofructo-1-kinase | GSD7 | PFK1 | PFKA | PFKX | Phosphofructokinase-M | Phosphofructokinase 1 | PFK-1 | PFK-A | Phosphofructokinase, muscle | Phosphohexokinase
Host
Goat
Reactivity
Rabbit (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Modifications
Liquid
Immunogen
Full length native Fructose-6-Phosphate Kinase purified from rabbit muscle.
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Fructose-6-Phosphate Kinase [Rabbit muscle]. Cross reactivity against Fructose-6-Phosphate Kinase from other sources is expected based on high degrees of sequence homology for muscle derived F6PK. Partial reactivity may occur against F6PK isolated from liver.
Applications
  • Immunofluorescence (1:200 - 1:2000)
  • Western blot (1:500 - 1:2000)
  • ELISA (1:1000 - 1:5000)
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PFKM / PFK-1
P08237 NM_000289 NP_000280.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/14/2021