Target
Rabbit PFKM / PFK-1
Synonyms
PFKM | 6-phosphofructo-1-kinase | GSD7 | PFK1 | PFKA | PFKX | Phosphofructokinase-M | Phosphofructokinase 1 | PFK-1 | PFK-A | Phosphofructokinase, muscle | Phosphohexokinase
Reactivity
Rabbit
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Modifications
Lyophilized
Immunogen
Full length native Fructose-6-Phosphate Kinase purified from rabbit muscle.
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Fructose-6-Phosphate Kinase [Rabbit muscle]. Cross reactivity against Fructose-6-Phosphate Kinase from other sources is expected based on high degrees of sequence homology for muscle derived F6PK. Partial reactivity may occur against F6PK isolated from liver.
Applications
- Immunofluorescence (1:200 - 1:2000)
- Western blot (1:500 - 1:2000)
- ELISA (1:1000 - 1:,5000)
Usage
FRUCTOSE-6-PHOSPHATE KINASE antibody has been tested for use in ELISA, immunofluorescence microscopy and western blot. Expect a band approximately 48 kD in size corresponding to the processed mature form of F6PK protein by western blotting in the appropriate cell lysate or extract.
Presentation
Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 100 µL of deionized water.
Storage
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee