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Catalog Number Size Price
LS-C209185-100 100 µg (1 mg/ml) $567 
PFKM / PFK-1 Antibody - Confocal microscopy images using anti-F6PK MAGP-2 antibody shows specific staining. Cultures of A7r5 VSM cells (rat aorta) were labeled with anti-F6PK (PFK) at 1:200 and anti-caveolin (CAV)-1. Images from labeled A7r5 cells were acquired separately for PFK fluorescence (green) and CAV-1 fluorescence (red) from each cell. Used with permission from Vallejo and Hardin (2004).

Polyclonal Goat anti‑Rabbit PFKM / PFK‑1 Antibody (Lyophilized, IF, WB) LS‑C209185

Polyclonal Goat anti‑Rabbit PFKM / PFK‑1 Antibody (Lyophilized, IF, WB) LS‑C209185

Antibody:
PFKM / PFK-1 Goat anti-Rabbit Polyclonal (Lyophilized) Antibody
Application:
IF, WB, ELISA
Reactivity:
Rabbit
Format:
Unconjugated, Lyophilized
Price
Catalog Number
$567
LS-C209185-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
PFKM / PFK-1 Goat anti-Rabbit Polyclonal (Lyophilized) Antibody
Application:
IF, WB, ELISA
Reactivity:
Rabbit
Format:
Unconjugated, Lyophilized

Specifications

Description
PFK-1 antibody LS-C209185 is an unconjugated goat polyclonal antibody to rabbit PFK-1 (PFKM). Validated for ELISA, IF and WB.
Target
Rabbit PFKM / PFK-1
Synonyms
PFKM | 6-phosphofructo-1-kinase | GSD7 | PFK1 | PFKA | PFKX | Phosphofructokinase-M | Phosphofructokinase 1 | PFK-1 | PFK-A | Phosphofructokinase, muscle | Phosphohexokinase
Host
Goat
Reactivity
Rabbit (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Modifications
Lyophilized
Immunogen
Full length native Fructose-6-Phosphate Kinase purified from rabbit muscle.
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Fructose-6-Phosphate Kinase [Rabbit muscle]. Cross reactivity against Fructose-6-Phosphate Kinase from other sources is expected based on high degrees of sequence homology for muscle derived F6PK. Partial reactivity may occur against F6PK isolated from liver.
Applications
  • Immunofluorescence (1:200 - 1:2000)
  • Western blot (1:500 - 1:2000)
  • ELISA (1:1000 - 1:,5000)
Usage
FRUCTOSE-6-PHOSPHATE KINASE antibody has been tested for use in ELISA, immunofluorescence microscopy and western blot. Expect a band approximately 48 kD in size corresponding to the processed mature form of F6PK protein by western blotting in the appropriate cell lysate or extract.
Presentation
Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 100 µL of deionized water.
Storage
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PFKM / PFK-1
P08237 NM_000289 NP_000280.1

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Requested From: United States
Date Requested: 4/25/2024