Target
Mouse PECAM-1 / CD31
Synonyms
PECAM1 | CD31 | CD31 antigen | EndoCAM | PECA1 | PECAM-1 | GPIIA
Reactivity
Mouse, Rat
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence C-EKTTIEYEVKVH, from the internal region of the protein sequence according to NP_032842.2NP_001027550.1.
Specificity
This antibody is expected to recognize both reported isoforms (NP_032842.2; NP_001027550.1).
Applications
- Western blot (0.3 - 1 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:128000)
Usage
Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 80-85kDa band observed in Mouse and Rat Spleen lysates (calculated MW of 80kDa according to Mouse NP_032842.2 and 76.2kDa according to Rat NP_113779.1). Recommended concentration: 0.3-1 ug/ml. Primary incubation 1 hour.at room temperature.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee