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PDX1 Antibody (phospho‑Thr11) LS‑C163661

Catalog Number Size Price
LS-C163661-400 400 µl $325 
Fluorescent confocal image of SY5Y cells stained with phospho-PDX1-T11 antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated phospho-PDX1-T11 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the phospho-PDX1 immunosignal mainly to the nucleus.
Dot blot of PDX1 Antibody (T11) antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
Fluorescent confocal image of SY5Y cells stained with phospho-PDX1-T11 antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated phospho-PDX1-T11 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the phospho-PDX1 immunosignal mainly to the nucleus.
Dot blot of PDX1 Antibody (T11) antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
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PDX1 Antibody (phospho‑Thr11) LS‑C163661

Antibody:
Rabbit Polyclonal to Human PDX1
Application:
IF, DB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$325 
LS-C163661-400
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human PDX1
Application:
IF, DB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
PDX1 antibody LS-C163661 is an unconjugated rabbit polyclonal antibody to human PDX1. Validated for DB and IF.
Target
Human PDX1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • Immunofluorescence (1:100)
  • Dot Blot (1:500)
Blocking Peptide
LS-E7453 - Lyophilized - 100 µg - $230.00
Immunizing peptide used to generate LS-C163661. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
pThr11
Specificity
This PDX1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T11 of human PDX1.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PDX1
P52945 NM_000209 NP_000200.1

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Requested From: United States
Date Requested: 8/24/2019
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