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Catalog Number Size Price
LS-C800879-100 100 µl (1 mg/ml) $379 
LS-C800879-200 200 µl (1 mg/ml) $421 
PDPK1 / PDK1 Antibody - 1:100 staining human appendix tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PDPK1 / PDK1 Antibody - 1:100 staining human gastric tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PDPK1 / PDK1 Antibody - 1:100 staining rat uterine tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PDPK1 / PDK1 Antibody - 1:100 staining human TB tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PDPK1 / PDK1 Antibody - 1:100 staining rat ovarian tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PDPK1 / PDK1 Antibody - 1:100 staining mouse brain tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PDPK1 / PDK1 Antibody - 1:100 staining mouse lung tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PDPK1 / PDK1 Antibody - 1:100 staining rat Intestinal tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PDPK1 / PDK1 Antibody - 1:100 staining mouse gastric tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PDPK1 / PDK1 Antibody - Western blot analysis of extracts of mouse brain tissue using Phospho-PDK1 (Ser241) antibody.
PDPK1 / PDK1 Antibody - Western blot analysis of PDK1 phosphorylation expression in EGF treated MDA-MB-435 whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
PDPK1 / PDK1 Antibody - Western blot analysis of PDK1 phosphorylation expression in mouse liver tissue lysates. The lane on the right is treated with the antigen-specific peptide.
PDPK1 / PDK1 Antibody - 1:100 staining human appendix tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PDPK1 / PDK1 Antibody - 1:100 staining human gastric tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PDPK1 / PDK1 Antibody - 1:100 staining rat uterine tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PDPK1 / PDK1 Antibody - 1:100 staining human TB tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PDPK1 / PDK1 Antibody - 1:100 staining rat ovarian tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PDPK1 / PDK1 Antibody - 1:100 staining mouse brain tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PDPK1 / PDK1 Antibody - 1:100 staining mouse lung tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PDPK1 / PDK1 Antibody - 1:100 staining rat Intestinal tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PDPK1 / PDK1 Antibody - 1:100 staining mouse gastric tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PDPK1 / PDK1 Antibody - Western blot analysis of extracts of mouse brain tissue using Phospho-PDK1 (Ser241) antibody.
PDPK1 / PDK1 Antibody - Western blot analysis of PDK1 phosphorylation expression in EGF treated MDA-MB-435 whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
PDPK1 / PDK1 Antibody - Western blot analysis of PDK1 phosphorylation expression in mouse liver tissue lysates. The lane on the right is treated with the antigen-specific peptide.
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Polyclonal Rabbit anti‑Human PDPK1 / PDK1 Antibody (phospho‑Ser241, IHC, WB) LS‑C800879

Polyclonal Rabbit anti‑Human PDPK1 / PDK1 Antibody (phospho‑Ser241, IHC, WB) LS‑C800879

Antibody:
PDPK1 / PDK1 Rabbit anti-Human Polyclonal (pSer241) Antibody
Application:
IHC, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C800879-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
PDPK1 / PDK1 Rabbit anti-Human Polyclonal (pSer241) Antibody
Application:
IHC, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
PDK1 antibody LS-C800879 is an unconjugated rabbit polyclonal antibody to PDK1 (PDPK1) (pSer241) from human. It is reactive with human, mouse and rat. Validated for IHC, Peptide-ELISA and WB.
Target
Human PDPK1 / PDK1
Synonyms
PDPK1 | HPDK1 | PkB kinase like gene 1 | PkB-like 1 | Pdk-1 | PkB kinase | PRO0461
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Modifications
Unmodified
Immunogen
A synthesized peptide derived from human PDK1 around the phosphorylation site of Serine 241.
Epitope
pSer241
Specificity
Phospho-PDK1 (Ser241) Antibody detects endogenous levels of PDK1 only when phosphorylated at Serine 241.
Applications
  • IHC (1:50 - 1:500)
  • Western blot (1:500 - 1:2000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PDPK1 / PDK1
O15530 NM_002613 NP_002604.1

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To request an SDS/MSDS form for this product, please contact our Technical Support department at:

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Requested From: United States
Date Requested: 4/24/2024