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PDPK1 / PDK1 Antibody (aa301-312) LS-C18965

PDK1 antibody LS-C18965 is an unconjugated rabbit polyclonal antibody to PDK1 (PDPK1) from human and rabbit. Validated for ELISA and WB.
Catalog
Size
Price
LS-C18965-100
100 µg (1.3 mg/ml)
$425
Description
PDK1 antibody LS-C18965 is an unconjugated rabbit polyclonal antibody to PDK1 (PDPK1) from human and rabbit. Validated for ELISA and WB.
Target
Rabbit PDPK1 / PDK1
Host
Rabbit
Reactivity
Rabbit, Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (1:500 - 1:2000)
  • ELISA (1:100000)
Failed Applications
  • IHC - Paraffin
Immunogen
PDPK1 / PDK1 antibody was raised against synthetic peptide from rabbit PDPK1.
Epitope
aa301-312
Specificity
Amino acids 301-312 of PDK-1 (genome data base accession number AC005591). Peptide sequence: R-K-I-Q-E-V-W-R-Q-R-Y-Q-S.
Usage
This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band ~60 kD in size corresponding to human PDK-1 protein by western blotting in the appropriate cell lysate or extract. Although untested, this reagent may be useful for immunohistochemistry and immunoprecipitation. Reactivity in other immunoassays is unknown.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About PDPK1 / PDK1
O15530 NM_002613 NP_002604.1

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Images

Western blot

Western blot of affinity purified anti-PKD-1 antibody shows detection of myc-tagged human PDK-1 at 60kD in ~10 ug of a virus infected Sf9 cell lysate (arrow). The nitrocellulose membrane was probed overnight at 4 degrees C with the primary antibody diluted 1:750 in 1% non-fat dry milk. HRP conjugated Goat-anti-Rabbit IgG and chemiluminescent detection is recommended. Other detection systems will yield similar results.
Western blot of affinity purified anti-PKD-1 antibody shows detection of myc-tagged human PDK-1 at 60kD in ~10 ug of a virus infected Sf9 cell lysate (arrow). The nitrocellulose membrane was probed overnight at 4 degrees C with the primary antibody diluted 1:750 in 1% non-fat dry milk. HRP conjugated Goat-anti-Rabbit IgG and chemiluminescent detection is recommended. Other detection systems will yield similar results.

Western blot

Western blot of affinity purified anti-PKD-1 antibody shows detection of myc-tagged human PDK-1 at 60kD in ~10 ug of a virus infected Sf9 cell lysate (arrow). The nitrocellulose membrane was probed overnight at 4 degrees C with the primary antibody diluted 1:750 in 1% non-fat dry milk. HRP conjugated Goat-anti-Rabbit IgG and chemiluminescent detection is recommended. Other detection systems will yield similar results.
Western blot of affinity purified anti-PKD-1 antibody shows detection of myc-tagged human PDK-1 at 60kD in ~10 ug of a virus infected Sf9 cell lysate (arrow). The nitrocellulose membrane was probed overnight at 4 degrees C with the primary antibody diluted 1:750 in 1% non-fat dry milk. HRP conjugated Goat-anti-Rabbit IgG and chemiluminescent detection is recommended. Other detection systems will yield similar results.

Western blot

Western blot of affinity purified anti-PKD-1 antibody shows detection of myc-tagged human PDK-1 at 60kD in ~10 ug of a virus infected Sf9 cell lysate (arrow). The nitrocellulose membrane was probed overnight at 4 degrees C with the primary antibody diluted 1:750 in 1% non-fat dry milk. HRP conjugated Goat-anti-Rabbit IgG and chemiluminescent detection is recommended. Other detection systems will yield similar results.
Western blot of affinity purified anti-PKD-1 antibody shows detection of myc-tagged human PDK-1 at 60kD in ~10 ug of a virus infected Sf9 cell lysate (arrow). The nitrocellulose membrane was probed overnight at 4 degrees C with the primary antibody diluted 1:750 in 1% non-fat dry milk. HRP conjugated Goat-anti-Rabbit IgG and chemiluminescent detection is recommended. Other detection systems will yield similar results.

Western blot

Western blot of affinity purified anti-PKD-1 antibody shows detection of myc-tagged human PDK-1 at 60kD in ~10 ug of a virus infected Sf9 cell lysate (arrow). The nitrocellulose membrane was probed overnight at 4 degrees C with the primary antibody diluted 1:750 in 1% non-fat dry milk. HRP conjugated Goat-anti-Rabbit IgG and chemiluminescent detection is recommended. Other detection systems will yield similar results.
Western blot of affinity purified anti-PKD-1 antibody shows detection of myc-tagged human PDK-1 at 60kD in ~10 ug of a virus infected Sf9 cell lysate (arrow). The nitrocellulose membrane was probed overnight at 4 degrees C with the primary antibody diluted 1:750 in 1% non-fat dry milk. HRP conjugated Goat-anti-Rabbit IgG and chemiluminescent detection is recommended. Other detection systems will yield similar results.

Requested From: United States
Date Requested: 4/22/2019
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