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Catalog Number Size Price
LS-C407984-10 10 µg $318 
LS-C407984-100 100 µg $470 
PDIA3 / ERp57 Antibody - IHC analysis of ERp57 using anti-ERp57 antibody. ERp57 was detected in frozen section of human placenta tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-ERp57 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
PDIA3 / ERp57 Antibody - ERp57 antibody IHC-paraffin. IHC(P): Human Lung Cancer Tissue.
PDIA3 / ERp57 Antibody - ERp57 antibody Western blot. All lanes: Anti ERp57 at 0.5 ug/ml. Lane 1: Rat Liver Tissue Lysate at 50 ug. Lane 2: Mouse Liver Tissue Lysate at 50 ug. Lane 3: A549 Whole Cell Lysate at 40 ug. Predicted band size: 57 kD. Observed band size: 57 kD.
PDIA3 / ERp57 Antibody - IHC analysis of ERp57 using anti-ERp57 antibody. ERp57 was detected in frozen section of human placenta tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-ERp57 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
PDIA3 / ERp57 Antibody - ERp57 antibody IHC-paraffin. IHC(P): Human Lung Cancer Tissue.
PDIA3 / ERp57 Antibody - ERp57 antibody Western blot. All lanes: Anti ERp57 at 0.5 ug/ml. Lane 1: Rat Liver Tissue Lysate at 50 ug. Lane 2: Mouse Liver Tissue Lysate at 50 ug. Lane 3: A549 Whole Cell Lysate at 40 ug. Predicted band size: 57 kD. Observed band size: 57 kD.
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Polyclonal Rabbit anti‑Human PDIA3 / ERp57 Antibody (aa471‑505, IHC, WB) LS‑C407984

Polyclonal Rabbit anti‑Human PDIA3 / ERp57 Antibody (aa471‑505, IHC, WB) LS‑C407984

Antibody:
PDIA3 / ERp57 Rabbit anti-Human Polyclonal (aa471-505) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Bat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C407984-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
PDIA3 / ERp57 Rabbit anti-Human Polyclonal (aa471-505) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Bat
Format:
Unconjugated, Unmodified

Specifications

Description
ERp57 antibody LS-C407984 is an unconjugated rabbit polyclonal antibody to ERp57 (PDIA3) (aa471-505) from human. It is reactive with human, mouse and bat. Validated for IHC and WB.
Target
Human PDIA3 / ERp57
Synonyms
PDIA3 | 58 kDa microsomal protein | ER protein 57 | ERp60 | ERp61 | Disulfide isomerase ER-60 | Endoplasmic reticulum P58 | GRP58 | HsT17083 | p58 | Phospholipase C-alpha | PI-PLC | ER protein 60 | ER60 | ERp57 | GRP57 | Protein disulfide-isomerase A3
Host
Rabbit
Reactivity
Human, Mouse, Bat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence at the C-Terminus of human ERp57 (471-505 aa RELSDFISYLQREATNPPVIQEEKPKKKKKAQEDL), different from the related mouse and rat sequences by two amino acids.
Epitope
aa471-505
Specificity
Detected in the flagellum and head region of spermatozoa (at protein level). .
Applications
  • IHC
  • IHC - Paraffin (0.5 - 1 µg/ml)
  • Western blot (0.1 - 0.5 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PDIA3 / ERp57
P30101 NM_005313 NP_005304.3

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/19/2024