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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PDGFRB / PDGFR Beta Antibody (phospho‑Tyr1021) LS‑C750620
PDGFR Beta antibody LS-C750620 is an unconjugated rabbit polyclonal antibody to PDGFR Beta (PDGFRB) from human, mouse and rat. Validated for WB.
Catalog
Size
Price
LS-C750620-50
50 µl
$265
Description
PDGFR Beta antibody LS-C750620 is an unconjugated rabbit polyclonal antibody to PDGFR Beta (PDGFRB) from human, mouse and rat. Validated for WB.
Target
Human PDGFRB / PDGFR Beta
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Applications
  • Western blot (1:500 - 1:2000)
Epitope
pTyr1021
Usage
The predicted MW is 37kDa/123kDa, while the observed MW by Western blot was 190kDa.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About PDGFRB / PDGFR Beta
P09619 NM_002609 NP_002600.1

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Images

Western blot

Western blot analysis of extracts of NIH/3T3 cells, using Phospho-PDGFRB-Y1021 antibody at 1:1000 dilution. NIH/3T3 cells were treated by PDGF (100ng/ml) for 30 minutes after serum-starvation overnight. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. Blocking buffer: 3% BSA.An ECL Kit was used for detection and the exposure time was 10s.
Western blot analysis of extracts of NIH/3T3 cells, using Phospho-PDGFRB-Y1021 antibody at 1:1000 dilution. NIH/3T3 cells were treated by PDGF (100ng/ml) for 30 minutes after serum-starvation overnight. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. Blocking buffer: 3% BSA.An ECL Kit was used for detection and the exposure time was 10s.

Western blot

Western blot analysis of extracts of NIH/3T3 cells, using Phospho-PDGFRB-Y1021 antibody at 1:1000 dilution. NIH/3T3 cells were treated by PDGF (100ng/ml) for 30 minutes after serum-starvation overnight. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. Blocking buffer: 3% BSA.An ECL Kit was used for detection and the exposure time was 10s.
Western blot analysis of extracts of NIH/3T3 cells, using Phospho-PDGFRB-Y1021 antibody at 1:1000 dilution. NIH/3T3 cells were treated by PDGF (100ng/ml) for 30 minutes after serum-starvation overnight. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. Blocking buffer: 3% BSA.An ECL Kit was used for detection and the exposure time was 10s.

Western blot

Western blot analysis of extracts of NIH/3T3 cells, using Phospho-PDGFRB-Y1021 antibody at 1:1000 dilution. NIH/3T3 cells were treated by PDGF (100ng/ml) for 30 minutes after serum-starvation overnight. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. Blocking buffer: 3% BSA.An ECL Kit was used for detection and the exposure time was 10s.
Western blot analysis of extracts of NIH/3T3 cells, using Phospho-PDGFRB-Y1021 antibody at 1:1000 dilution. NIH/3T3 cells were treated by PDGF (100ng/ml) for 30 minutes after serum-starvation overnight. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. Blocking buffer: 3% BSA.An ECL Kit was used for detection and the exposure time was 10s.

Western blot

Western blot analysis of extracts of NIH/3T3 cells, using Phospho-PDGFRB-Y1021 antibody at 1:1000 dilution. NIH/3T3 cells were treated by PDGF (100ng/ml) for 30 minutes after serum-starvation overnight. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. Blocking buffer: 3% BSA.An ECL Kit was used for detection and the exposure time was 10s.
Western blot analysis of extracts of NIH/3T3 cells, using Phospho-PDGFRB-Y1021 antibody at 1:1000 dilution. NIH/3T3 cells were treated by PDGF (100ng/ml) for 30 minutes after serum-starvation overnight. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. Blocking buffer: 3% BSA.An ECL Kit was used for detection and the exposure time was 10s.

Requested From: United States
Date Requested: 12/14/2018
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