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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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PDGF‑CC Antibody (aa230‑345) LS‑C150028

Catalog Number Size Price
LS-C150028-25 25 µg $255 
LS-C150028-100 100 µg (0.2 mg/ml) $530 
PDGF-C in Human Pancreatic Cancer Tissue. PDGF-C was detected in immersion fixed paraffin-embedded sections of human pancreatic cancer tissue using 1.7 ug/ml Goat Anti-Human PDGF-C Antigen Affinity-purified Polyclonal Antibody overnight at 4°C. Tissue was stained with Anti-Goat HRP-DAB (brown) and counterstained with hematoxylin (blue).
Detection of Human PDGFC by Western Blot. Western blot shows lysates of human platelets. PVDF membrane was probed with 1 ug/ml of Goat Anti-Human PDGFC Antigen Affinity-purified Polyclonal Antibody followed by HRP-conjugated Anti-Goat IgG Secondary Antibody. Specific bands were detected for PDGF Cp80 atapproximately 80 kD and PDGFC p55 at approximately 55 kD (as indicated). This experiment was conducted under reducing conditions.
Cell Proliferation Induced by PDGF-CC and Neutralization by Human PDGF-C Antibody. Recombinant Human PDGF-CC stimulates proliferation in the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human PDGF-CC (0.8 ug/ml) is neutralized (green line) by increasing concentrations of Goat Anti-Human PDGF-C Antigen Affinity-purified Polyclonal Antibody. The ND50 is typically 6-24 ug/ml.
PDGF-C in Human Pancreatic Cancer Tissue. PDGF-C was detected in immersion fixed paraffin-embedded sections of human pancreatic cancer tissue using 1.7 ug/ml Goat Anti-Human PDGF-C Antigen Affinity-purified Polyclonal Antibody overnight at 4°C. Tissue was stained with Anti-Goat HRP-DAB (brown) and counterstained with hematoxylin (blue).
Detection of Human PDGFC by Western Blot. Western blot shows lysates of human platelets. PVDF membrane was probed with 1 ug/ml of Goat Anti-Human PDGFC Antigen Affinity-purified Polyclonal Antibody followed by HRP-conjugated Anti-Goat IgG Secondary Antibody. Specific bands were detected for PDGF Cp80 atapproximately 80 kD and PDGFC p55 at approximately 55 kD (as indicated). This experiment was conducted under reducing conditions.
Cell Proliferation Induced by PDGF-CC and Neutralization by Human PDGF-C Antibody. Recombinant Human PDGF-CC stimulates proliferation in the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human PDGF-CC (0.8 ug/ml) is neutralized (green line) by increasing concentrations of Goat Anti-Human PDGF-C Antigen Affinity-purified Polyclonal Antibody. The ND50 is typically 6-24 ug/ml.
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PDGF‑CC Antibody (aa230‑345) LS‑C150028

Antibody:
Goat Polyclonal to Human PDGF-CC
Application:
IHC, IHC-P, WB, Neut
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$255 
LS-C150028-25
Toll Free North America
866-819-4732

Overview

Antibody:
Goat Polyclonal to Human PDGF-CC
Application:
IHC, IHC-P, WB, Neut
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
PDGF-CC antibody LS-C150028 is an unconjugated goat polyclonal antibody to human PDGF-CC. Validated for IHC, Neut and WB.
Target
Human PDGF-CC
Host
Goat
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 - 15 µg/ml)
  • Western blot (1 µg/ml)
  • Neutralization
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
E. coli-derived recombinant human PDGFC Gly230-Gly345 Accession # Q9-NRA1.
Epitope
aa230-345
Specificity
Detects human PDGFC in direct ELISAs and Western blots. In Western blots, approximately 15% cross-reactivity with recombinant mouse.
Usage
Western Blot, 1 µg/ml. Immunohistochemistry, 5-15 µg/ml. Neutralization, Measured by its ability to neutralize PDGF-CC-induced proliferation in the NR6R-3T3 mouse fibroblast cell line. The Neutralization Dose (ND50) is typically 6-24 µg/ml in the presence of 0.8 µg/ml Recombinant Human PDGF-CC.
Presentation
Lyophilized from PBS, pH 7.4, 5% Trehalose
Reconstitution
Reconstitute with 500uL of sterile PBS.
Storage
Store at -20°C to -70°C for up to 1 year.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PDGF-CC
Q9NRA1 NM_016205 NP_057289.1

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Requested From: United States
Date Requested: 8/18/2019
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