PDGF-AA antibody LS-C17315 is an unconjugated goat polyclonal antibody to PDGF-AA from human, bovine, pig and other species. Validated for Func and WB.
Human, Bovine, Pig, Rodent
(tested or 100% immunogen sequence identity)
Also available conjugated with AP
Ion exchange chromatography
- Western blot (5 - 10 µg/ml)
- Functional Assay
- (applications tested for the base form of this product only)
PDGF-AA antibody was raised against purified human PDGF-AB.
Recognizes non-reduced human PDGF AA, AB and BB dimers in western immunoblots. Porcine and bovine PDGF may be detected with decreased sensitivity. Rodent PDGF will not be detected. This antibody will neutralize human, porcine, bovine and rodent PDGF.
Suitable for use in Western Blot and Growth Inhibition Assays. 2. Prepare a 3T3 cell suspension at 10^4 cells/ml and aliquot 100 ul to each well. 3. Refeed on Day 3. 4. Count wells on Day 5 using an ATP endpoint assay. Wash cell layer once with PBS prior to lysis.
0.1 M Tris-glycine, pH 7.0, 40% glycerol. No preservative added.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.