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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PDE2A Antibody (N‑Terminus) IHC‑plus™ LS‑A733 Best Seller
PDE2A antibody LS-A733 is an unconjugated rabbit polyclonal antibody to PDE2A from human, dog and horse. Validated for IHC. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-A733-50
50 µg (1 mg/ml)
$425
In Stock for Immediate Shipment

Popular PDE2A Products

Western blot of PDE2A antibody (1:2,000) against lysate from COS cells transfected with the indicated human PDE isoform.
Species: Human, Mouse, Rat
Applications: IHC, Western blot, Immunoprecipitation
Anti-PDE2 / PDE2A antibody IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10450 dilution 1:50.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDE2A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDE2A.
Species: Human
Applications: Western blot, Flow Cytometry
IHC of paraffin-embedded Human Kidney tissue using anti-PDE2A mouse monoclonal antibody.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry

Product Description

PDE2A antibody LS-A733 is an unconjugated rabbit polyclonal antibody to PDE2A from human, dog and horse. Validated for IHC. Tested on 20 paraffin-embedded human tissues.

Specifications

Target
Human PDE2A
Synonyms
PDE2A, CGMP-stimulated PDE, Cyclic GMP-stimulated PDE, PDE2, Pde2a2, PED2A4, Phosphodiesterase 2A, CGS-PDE, CGSPDE, PDE2A1, Pde2a3
Host
Rabbit
Reactivity
Human, Dog, Horse (tested or 100% immunogen sequence identity)
Predicted
Monkey, Mouse, Rat, Bovine, Rabbit (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (20 µg/ml)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
PDE2A antibody was raised against synthetic 17 amino acid peptide from N-terminus of human PDE2A. Percent identity with other species by BLAST analysis: Human, Gorilla, Marmoset, Dog, Panda, Horse (100%); Gibbon, Monkey, Mouse, Rat, Bovine, Elephant, Rabbit (94%); Pig (88%).
Blocking Peptide
LS-E29255 - Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-A733. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
N-Terminus
Specificity
Human PDE2A. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except KCNH2 (59%).
Usage
Immunohistochemistry: LS-A733 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-A733 was determined to be 20 ug/ml.
Presentation
PBS, 0.1% Sodium Azide
Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About PDE2A
Phosphodiesterase 2A is a cGMP stimulated, cAMP/cGMP dual-specific phosphodiesterase whose hydrolytic activity is stimulated by the presence of cGMP binding to the GAF domain. PDE2A has been implicated in penile erectile dysfunction and in the regulation of fluid and inflammatory cell transit across the endothelial cell barrier (i. e., venous and capillary). PDE2A activity is inhibited by erythro-9-(2-hydroxyl-3-nonyl)adenine (EHNA). O00408 NM_002599 NP_002590.1


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Immunohistochemistry

Anti-PDE2A antibody LS-A733 IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-PDE2A antibody LS-A733 IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-PDE2A antibody LS-A733 IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-PDE2A antibody LS-A733 IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-PDE2A antibody LS-A733 IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-PDE2A antibody LS-A733 IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-PDE2A antibody LS-A733 IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-PDE2A antibody LS-A733 IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Requested From: United States
Date Requested: 10/19/2018

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