Products
Research Areas
COVID-19
Resources
Contact Us
Distributors
Login
Quick Order
Cart
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Contact Us
LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121
Phone:
866-819-4732 (Toll Free North America
206-374-1102 (International)
Fax:
866-206-6909 (Toll Free North America)
206-577-4565 (International)
How To Buy - Details about how to buy our products.
Orders@LSBio.com - To submit a new order.
Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, Proforma invoice requests, or other billing issues.
Technical.Support@LSBio.com - To request technical information about an LSBio product or its applications
Sales@LSBio.com - To request information about distribution agreements, or general business development.
Worldwide Distributors List - To find your local distributor if you're not within the United States.
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Catalog Number Size Price
LS-C745302-25 25 µl (1 mg/ml) $275 
PDCD4 Antibody - Affinity purified anti-Pdcd4 pS457 antibody was used at 1.25 µg/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderate positive staining of human breast epithelial cells at 40X. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
PDCD4 Antibody - Western blot using the affinity purified anti-Pdcd4 pS457 antibody shows detection of Pdcd4 phosphorylated at Ser 457 (arrowheads). Lanes 1 & 2 each contain 100 ng recombinant Pdcd4. Lanes 3 & 4 each contain 30 µg of whole cell extract from 293 HEK cells treated with 20 nM TPA and MG132 proteosome inhibitor for 8 hours. The signal can be competed off with peptide phosphorylated at Ser 457 (Lanes 2 & 4).
PDCD4 Antibody - Affinity purified anti-Pdcd4 pS457 antibody was used at 1.25 µg/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderate positive staining of human breast epithelial cells at 40X. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
PDCD4 Antibody - Western blot using the affinity purified anti-Pdcd4 pS457 antibody shows detection of Pdcd4 phosphorylated at Ser 457 (arrowheads). Lanes 1 & 2 each contain 100 ng recombinant Pdcd4. Lanes 3 & 4 each contain 30 µg of whole cell extract from 293 HEK cells treated with 20 nM TPA and MG132 proteosome inhibitor for 8 hours. The signal can be competed off with peptide phosphorylated at Ser 457 (Lanes 2 & 4).
1 of 2
2 of 2

Polyclonal Rabbit anti‑Human PDCD4 Antibody (phospho‑Ser457, IHC, WB) LS‑C745302

Polyclonal Rabbit anti‑Human PDCD4 Antibody (phospho‑Ser457, IHC, WB) LS‑C745302

Antibody:
PDCD4 Rabbit anti-Human Polyclonal (pSer457) Antibody
Application:
IHC, WB, ELISA
Reactivity:
Human, Mouse, Rat, Xenopus
Format:
Unconjugated, Unmodified
Price
Catalog Number
$275
LS-C745302-25
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
PDCD4 Rabbit anti-Human Polyclonal (pSer457) Antibody
Application:
IHC, WB, ELISA
Reactivity:
Human, Mouse, Rat, Xenopus
Format:
Unconjugated, Unmodified

Specifications

Description
PDCD4 antibody LS-C745302 is an unconjugated rabbit polyclonal antibody to PDCD4 (pSer457) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, IHC and WB.
Target
Human PDCD4
Synonyms
PDCD4 | H731 | Nuclear antigen H731-like | Nuclear antigen H731 | Protein 197/15a
Host
Rabbit
Reactivity
Human, Mouse, Rat, Xenopus (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat, Xenopus (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Anti-Pdcd4 pS457 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids surrounding Ser457 in the human Pdcd4 protein.
Epitope
pSer457
Specificity
This antibody is specific for human Pdcd4 protein phosphorylated at Ser457. A BLAST analysis was used to suggest cross-reactivity with Pdcd4 from human, mouse, rat and Xenopus based on 100% homology with the immunizing sequence. Cross-reactivity with Pdcd4 from other sources has not been determined.
Applications
  • IHC (1.25 - 2.5 µg/ml)
  • Western blot (1:500 - 1:2000)
  • ELISA (1:400000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PDCD4
Q53EL6 NM_014456 NP_055271.2

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/14/2021