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Catalog Number	Size	Price
LS-C744821-25	25 µl (1 mg/ml)	$304

PDCD4 Antibody - Antibody 200-301-964 has been tested in immunohistochemistry, analyzed by an anatomic pathologist and validated for use in IHC applications against formalin-fixed, paraffin-embedded human tissues. The antibody was serially diluted and tested at a range of concentrations on at least 22 different human formalin-fixed, paraffin archival tissues, and positive and negative tissues were scored and compared to the published literature on the expression and function of the gene. A representative image from positively stained small intestine shows the localization of the anti Pdcd4 antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.

PDCD4 Antibody - Western blot using Protein A purified Mouse Monoclonal anti-Pdcd4 pS457 antibody against recombinant PDCD4 protein. Membrane was blocked in 1% BSA-TBS-T for 30 min RT and probed with 1° Ab Ms-A-Pdcd4pS457 1:1000 (o/n 4°C in 1% BSA-TBS-T) followed by 2° Ab Peroxidase Conjugated Rabbit anti Ms CUST10M at 1:40,000 in MB-070 30 min RT. Bands at ~62 kD and ~32 kD were detected.

PDCD4 Antibody - Western blot using the Protein A purified Mouse Monoclonal anti-Pdcd4 pS457 antibody shows detection of phosphorylated Pdcd4 (indicated by arrowhead at ~62 kDa) in NIH-3T3 cells after 5 min treatment with 30 ng/mL PDGF (lane 2). No reactivity is seen for unstimulated (non-phosphorylated) NIH 3T3 cells (lane 1). The membrane was probed with the primary antibody at a 1:2,000 dilution, overnight at 4° C. For detection HRP conjugated Rb-a-Mouse IgG was used at a 1:20,000 dilution in blocking buffer for 1 h at 4° C followed by visualization using a Biospectrum imaging system (UVP).

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Monoclonal Mouse anti‑Human PDCD4 Antibody (clone 9G6, phospho‑Ser457, IHC, WB) LS‑C744821

Name: Anti-PDCD4 Antibody | Mouse anti-Human Monoclonal ELISA,IHC,WB | LSBio
Brand: Lifespan BioSciences
SKU: LS-C744821-25
Price: 304.0000 USD
Availability: InStock

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Antibody:

PDCD4 Mouse anti-Human Monoclonal (pSer457) (9G6) Antibody

Application:

IHC, WB, ELISA

Reactivity:

Human, Mouse, Rat, Xenopus

Format:

Unconjugated, Unmodified

$304

LS-C744821-25

Toll Free North America

206-374-1102

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

PDCD4 Mouse anti-Human Monoclonal (pSer457) (9G6) Antibody

Application:

IHC, WB, ELISA

Reactivity:

Human, Mouse, Rat, Xenopus

Format:

Unconjugated, Unmodified

Specifications

Description

PDCD4 antibody LS-C744821 is an unconjugated mouse monoclonal antibody to PDCD4 (pSer457) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, IHC and WB.

Target

Human PDCD4

Synonyms

PDCD4 | H731 | Nuclear antigen H731-like | Nuclear antigen H731 | Protein 197/15a

See All PDCD4 Antibodies

Host

Mouse

Reactivity

Human, Mouse, Rat, Xenopus (tested or 100% immunogen sequence identity)

Clonality

IgG1,k Monoclonal

Clone

9G6

Conjugations

Unconjugated

Purification

Protein A affinity chromatography

Modifications

Unmodified

Immunogen

This monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding Ser457 of the human Pdcd4 protein.

Epitope

pSer457

Specificity

This antibody is specific for human Pdcd4 protein phosphorylated at Ser457. A BLAST analysis was used to suggest cross-reactivity with Pdcd4 from human, mouse, rat and Xenopus based on 100% homology with the immunizing sequence. Cross-reactivity with Pdcd4 from other sources has not been determined.

Applications

IHC (1:1000 - 1:5000)
Western blot (1:2000 - 1:10000)
ELISA (1:20000 - 1:100000)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Usage

Applications should be user optimized.

Presentation

0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Storage

Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About PDCD4

Uniprot: Q53EL6 NCBI: NM_014456 NP_055271.2