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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-C154004-100 100 µg (1 mg/ml) $425 
PDCD4 Antibody - Anti-Pdcd4 Antibody - Immunohistochemistry. Antibody Anti-Pdcd4 pS457 (MOUSE) Monoclonal Antibody - 200-301-964 has been tested in immunohistochemistry, analyzed by an anatomic pathologist and validated for use in IHC applications against formalin-fixed, paraffin-embedded human tissues. The antibody was serially diluted and tested at a range of concentrations on at least 22 different human formalin-fixed, paraffin archival tissues, and positive and negative tissues were scored and compared to the published literature on the expression and function of the gene. A representative image from positively stained small intestine shows the localization of the anti-Pdcd4 antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Image provided courtesy of LifeSpan Biosciences, Seattle, WA.
PDCD4 Antibody - PDCD4 antibody Western Blot. Western blot of Immunochemicals Protein A purified Mouse Monoclonal anti-Pdcd4 pS457 antibody against recombinant PDCD4 protein. Membrane was blocked in 1% BSA-TBS-T for 30 min RT and probed with 1? Ab Ms-A-Pdcd4pS457 1:1000 (o/n 4C in 1% BSA-TBS-T) followed by 2? Ab Peroxidase Conjugated Rabbit anti-Ms CUST10M Lot# 20121 at 1:40000 in MB-070 30 min RT. Bands at ~62 kD and ~32 kD were detected.
PDCD4 Antibody - Anti-Pdcd4 pS457 Antibody - Western Blot. Western blot of Protein A purified Mouse Monoclonal anti-Pdcd4 pS457 antibody shows detection of phosphorylated Pdcd4 (indicated by arrowhead at ~62 kD) in NIH-3T3 cells after 5 min treatment with 30 ng/mL PDGF (lane 2). No reactivity is seen for unstimulated (non-phosphorylated) NIH 3T3 cells (lane 1). The membrane was probed with the primary antibody at a 1:2000 dilution, overnight at 4C. For detection HRP conjugated Rb-a-Mouse IgG was used at a 1:20000 dilution in blocking buffer (MB-070) for 1 h at 4C followed by visualization using a Biospectrum imaging system (UVP).
PDCD4 Antibody - Anti-Pdcd4 Antibody - Immunohistochemistry. Antibody Anti-Pdcd4 pS457 (MOUSE) Monoclonal Antibody - 200-301-964 has been tested in immunohistochemistry, analyzed by an anatomic pathologist and validated for use in IHC applications against formalin-fixed, paraffin-embedded human tissues. The antibody was serially diluted and tested at a range of concentrations on at least 22 different human formalin-fixed, paraffin archival tissues, and positive and negative tissues were scored and compared to the published literature on the expression and function of the gene. A representative image from positively stained small intestine shows the localization of the anti-Pdcd4 antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Image provided courtesy of LifeSpan Biosciences, Seattle, WA.
PDCD4 Antibody - PDCD4 antibody Western Blot. Western blot of Immunochemicals Protein A purified Mouse Monoclonal anti-Pdcd4 pS457 antibody against recombinant PDCD4 protein. Membrane was blocked in 1% BSA-TBS-T for 30 min RT and probed with 1? Ab Ms-A-Pdcd4pS457 1:1000 (o/n 4C in 1% BSA-TBS-T) followed by 2? Ab Peroxidase Conjugated Rabbit anti-Ms CUST10M Lot# 20121 at 1:40000 in MB-070 30 min RT. Bands at ~62 kD and ~32 kD were detected.
PDCD4 Antibody - Anti-Pdcd4 pS457 Antibody - Western Blot. Western blot of Protein A purified Mouse Monoclonal anti-Pdcd4 pS457 antibody shows detection of phosphorylated Pdcd4 (indicated by arrowhead at ~62 kD) in NIH-3T3 cells after 5 min treatment with 30 ng/mL PDGF (lane 2). No reactivity is seen for unstimulated (non-phosphorylated) NIH 3T3 cells (lane 1). The membrane was probed with the primary antibody at a 1:2000 dilution, overnight at 4C. For detection HRP conjugated Rb-a-Mouse IgG was used at a 1:20000 dilution in blocking buffer (MB-070) for 1 h at 4C followed by visualization using a Biospectrum imaging system (UVP).
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Monoclonal PDCD4 Antibody (clone 9G6, phospho‑Ser457, IHC, WB) LS‑C154004

Monoclonal PDCD4 Antibody (clone 9G6, phospho‑Ser457, IHC, WB) LS‑C154004

Antibody:
Mouse Monoclonal to Human PDCD4
Application:
IHC, WB, ELISA
Reactivity:
Human, Mouse, Rat, Xenopus
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-C154004-100
100 µg
1 mg/ml
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Mouse Monoclonal to Human PDCD4
Application:
IHC, WB, ELISA
Reactivity:
Human, Mouse, Rat, Xenopus
Format:
Unconjugated, Unmodified

Specifications

Description
PDCD4 antibody LS-C154004 is an unconjugated mouse monoclonal antibody to PDCD4 (pSer457) from human, mouse, rat and other species. Validated for ELISA, IHC and WB. Cited in 1 publication.
Target
Human PDCD4
Synonyms
PDCD4 | H731 | Nuclear antigen H731-like | Nuclear antigen H731 | Protein 197/15a
Host
Mouse
Reactivity
Human, Mouse, Rat, Xenopus (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Clone
9G6
Conjugations
Unconjugated
Purification
Protein A affinity chromatography
Modifications
Unmodified
Applications
  • IHC (1:1000 - 1:5000)
  • Western blot (1:2000 - 1:10000)
  • ELISA (1:20000 - 1:100000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
This monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding Ser457 of the human Pdcd4 protein.
Epitope
pSer457
Specificity
This antibody is specific for human Pdcd4 protein phosphorylated at Ser457. A BLAST analysis was used to suggest cross-reactivity with Pdcd4 from human, mouse, rat and Xenopus based on 100% homology with the immunizing sequence. Cross-reactivity with Pdcd4 from other sources has not been determined.
Usage
Pdcd4 phospho S457 Antibody is suitable for ELISA, immunohistochemistry and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 62 kD in size corresponding to phosphorylated Pdcd4 protein by western blotting in the appropriate cell lysate or extract. This phospho-specific monoclonal antibody reacts with human Pdcd4 pS457 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PDCD4
Q53EL6 NM_014456 NP_055271.2

LSBio Ratings

PDCD4 Antibody (phospho-Ser457, clone 9G6) for IHC, WB/Western, ELISA LS-C154004 has an LSBio Rating of
Publications (4)

Publications (1)

Up-regulation of miR-21 by HER2/neu signaling promotes cell invasion. Huang TH, Wu F, Loeb GB, Hsu R, Heidersbach A, Brincat A, Horiuchi D, Lebbink RJ, Mo YY, Goga A, McManus MT. The Journal of biological chemistry. 2009 284:18515-24.

Customer Reviews (0)


Requested From: United States
Date Requested: 4/5/2020