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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

Research Areas
Cell Cycle Pathways
Protein Family And Group
Infectious Disease
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Catalog Number Size Price
LS-C153791-50 50 µg (0.5 mg/ml) $365 
PDCD4 Antibody - Western blot of Mouse anti-Pdcd4 phospho S457 Biotin Conjugated antibody. Lane 1: Pdcd4 recombinant protein. Lane 2: none. Load: 100 ng per lane. Primary antibody: Pdcd4 phospho S457 Biotin Conjugated antibody at 1:1000 for overnight at 4C. Secondary antibody: HRP Streptavidin secondary antibody at 1:40000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 50 kDa, 50 kDa for Pdcd4 phospho S457. Other band(s): Pdcd4 phospho S457 splice variants and isoforms This image was taken for the unconjugated form of this product. Other forms have not been tested.

Monoclonal Mouse anti‑Human PDCD4 Antibody (clone 9G6, Biotin, phospho‑Ser457, WB) LS‑C153791

Monoclonal Mouse anti‑Human PDCD4 Antibody (clone 9G6, Biotin, phospho‑Ser457, WB) LS‑C153791

Antibody:
Mouse Monoclonal to Human PDCD4
Application:
WB, IP, ELISA
Reactivity:
Human, Mouse, Rat, Xenopus
Format:
Biotin, Unmodified
Price
Catalog Number
$365 
LS-C153791-50
50 µg
0.5 mg/ml
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Mouse Monoclonal to Human PDCD4
Application:
WB, IP, ELISA
Reactivity:
Human, Mouse, Rat, Xenopus
Format:
Biotin, Unmodified

Specifications

Description
PDCD4 antibody LS-C153791 is a biotin-conjugated mouse monoclonal antibody to PDCD4 (pSer457) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, IP and WB. Cited in 1 publication.
Target
Human PDCD4
Synonyms
PDCD4 | H731 | Nuclear antigen H731-like | Nuclear antigen H731 | Protein 197/15a
Host
Mouse
Reactivity
Human, Mouse, Rat, Xenopus (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Clone
9G6
Conjugations
Biotin
Purification
Protein A affinity chromatography
Modifications
Unmodified
Applications
  • Western blot (1:2000 - 1:10000)
  • Immunoprecipitation (1:1000 - 1:5000)
  • ELISA (1:20000 - 1:100000)
Immunogen
This monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding Ser457 of the human Pdcd4 protein.
Epitope
pSer457
Specificity
This antibody is specific for human Pdcd4 protein phosphorylated at Ser457. A BLAST analysis was used to suggest cross-reactivity with Pdcd4 from human, mouse, rat and Xenopus based on 100% homology with the immunizing sequence. Cross-reactivity with Pdcd4 from other sources has not been determined.
Usage
Anti-Pdcd4 pS457 (MOUSE) Monoclonal Antibody Biotin Conjugated is suitable for ELISA, immunohistochemistry and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 62 kD in size corresponding to phosphorylated Pdcd4 protein by western blotting in the appropriate cell lysate or extract. This phospho-specific monoclonal antibody reacts with human Pdcd4 pS457 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 100 µL of deionized water.
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PDCD4
Q53EL6 NM_014456 NP_055271.2

LSBio Ratings

PDCD4 Antibody (phospho-Ser457, clone 9G6, Biotin) for WB/Western, IP, ELISA LS-C153791 has an LSBio Rating of
Publications (4)

Publications (1)

Up-regulation of miR-21 by HER2/neu signaling promotes cell invasion. Huang TH, Wu F, Loeb GB, Hsu R, Heidersbach A, Brincat A, Horiuchi D, Lebbink RJ, Mo YY, Goga A, McManus MT. The Journal of biological chemistry. 2009 284:18515-24.

Customer Reviews (0)


Requested From: United States
Date Requested: 5/24/2020