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Catalog Number Size Price
LS-C745303-25 25 µl (1 mg/ml) $275 
PDCD4 Antibody - Affinity purified anti-Pdcd4 was used at a 1:100 dilution to detect Pdcd4 by immunohistochemistry on mouse colon tissue. Tissue was fixed in 4% paraformaldehyde and paraffin embedded. Tissue sections were deparaffinized and treated by trypsinization before staining.
PDCD4 Antibody - Western blot using the affinity purified anti-Pdcd4 antibody shows detection of a band ~52 kDa in size corresponding to Pdcd4 (arrowhead). Lane 1 contains recombinant Pdcd4. Lane 2 contains 293 HEK cells treated with TPA and MG132. The anti-Pdcd4 antibody was used at a 1: 5,000 dilution.
PDCD4 Antibody - Western Blot of rabbit anti-PDCD antibody. Marker: Opal Pre-stained ladder Lane 1: HEK293 lysate Lane 2: HeLa Lysate Lane 3: MCF-7 Lysate Lane 4: Jurkat Lysate Lane 5: A431 Lysate Lane 6: Raji Lsyate Lane 7: Ramos Lysate Lane 8: NIH/3T3 Lysate Load: 35 µg per lane. Primary antibody: PDCD antibody at 1:1,000 for 3hrs at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:30,000 for 60 min at RT. Blocking Buffer: 1% Casein-TTBS for 30 min at RT. Predicted/Observed size: 52 kDa for PDCD.
PDCD4 Antibody - Affinity purified anti-Pdcd4 was used at a 1:100 dilution to detect Pdcd4 by immunohistochemistry on mouse colon tissue. Tissue was fixed in 4% paraformaldehyde and paraffin embedded. Tissue sections were deparaffinized and treated by trypsinization before staining.
PDCD4 Antibody - Western blot using the affinity purified anti-Pdcd4 antibody shows detection of a band ~52 kDa in size corresponding to Pdcd4 (arrowhead). Lane 1 contains recombinant Pdcd4. Lane 2 contains 293 HEK cells treated with TPA and MG132. The anti-Pdcd4 antibody was used at a 1: 5,000 dilution.
PDCD4 Antibody - Western Blot of rabbit anti-PDCD antibody. Marker: Opal Pre-stained ladder Lane 1: HEK293 lysate Lane 2: HeLa Lysate Lane 3: MCF-7 Lysate Lane 4: Jurkat Lysate Lane 5: A431 Lysate Lane 6: Raji Lsyate Lane 7: Ramos Lysate Lane 8: NIH/3T3 Lysate Load: 35 µg per lane. Primary antibody: PDCD antibody at 1:1,000 for 3hrs at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:30,000 for 60 min at RT. Blocking Buffer: 1% Casein-TTBS for 30 min at RT. Predicted/Observed size: 52 kDa for PDCD.
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Polyclonal Rabbit anti‑Human PDCD4 Antibody (IHC, WB) LS‑C745303

Polyclonal Rabbit anti‑Human PDCD4 Antibody (IHC, WB) LS‑C745303

Antibody:
PDCD4 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, WB, ELISA
Reactivity:
Human, Mouse, Rat, Xenopus
Format:
Unconjugated, Unmodified
Price
Catalog Number
$275
LS-C745303-25
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
PDCD4 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, WB, ELISA
Reactivity:
Human, Mouse, Rat, Xenopus
Format:
Unconjugated, Unmodified

Specifications

Description
PDCD4 antibody LS-C745303 is an unconjugated rabbit polyclonal antibody to PDCD4 from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, IHC and WB.
Target
Human PDCD4
Synonyms
PDCD4 | H731 | Nuclear antigen H731-like | Nuclear antigen H731 | Protein 197/15a
Host
Rabbit
Reactivity
Human, Mouse, Rat, Xenopus (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat, Xenopus (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding amino acids near the carboxyl terminus of human Pdcd4 protein.
Specificity
A BLAST analysis was used to suggest cross-reactivity with Pdcd4 from human, mouse, rat and Xenopus based on 100% homology with the immunizing sequence. Cross-reactivity with Pdcd4 from other sources has not been determined. The antibody reacts with Pdcd4 protein that is either phosphorylated or non-phosphorylated at Ser457.
Applications
  • IHC (1:100 - 1:300)
  • Western blot (1:1000 - 1:10000)
  • ELISA (1:10000 - 1:120000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PDCD4
Q53EL6 NM_014456 NP_055271.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/10/2021